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Development and evaluation of a core genome multilocus sequence typing scheme for Paenibacillus larvae, the deadly American foulbrood pathogen of honeybees

Bertolotti, Alicia C. and Forsgren, Eva and Schaefer, Marc O. and Sircoulomb, Fabrice and Gaiani, Nicolas and Ribiere-Chabert, Magali and Paris, Laurianne and Lucas, Pierrick and de Boisséson, Claire and Skarin, Joakim and Riviere, Marie-Pierre (2021). Development and evaluation of a core genome multilocus sequence typing scheme for Paenibacillus larvae, the deadly American foulbrood pathogen of honeybees. Environmental Microbiology
[Research article]

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Abstract

Paenibacillus larvae is the causative agent of the fatal American foulbrood disease in honeybees (Apis mellifera). Strain identification is vital for preventing the spread of the disease. To date, the most accessible and robust scheme to identify strains is the multilocus sequence typing (MLST) method. However, this approach has limited resolution, especially for epidemiological studies. As the cost of whole-genome sequencing has decreased and as it becomes increasingly available to most laboratories, an extended MLST based on the core genome (cgMLST) presents a valuable tool for high-resolution investigations. In this study, we present a standardized, robust cgMLST scheme for P. larvae typing using whole-genome sequencing. A total of 333 genomes were used to identify, validate and evaluate 2419 core genes. The cgMLST allowed fine-scale differentiation between samples that had the same profile using traditional MLST and allowed for the characterization of strains impossible by MLST. The scheme was successfully used to trace a localized Swedish outbreak, where a cluster of 38 isolates was linked to a country-wide beekeeping operation. cgMLST greatly enhances the power of a traditional typing scheme, while preserving the same stability and standardization for sharing results and methods across different laboratories.

Authors/Creators:Bertolotti, Alicia C. and Forsgren, Eva and Schaefer, Marc O. and Sircoulomb, Fabrice and Gaiani, Nicolas and Ribiere-Chabert, Magali and Paris, Laurianne and Lucas, Pierrick and de Boisséson, Claire and Skarin, Joakim and Riviere, Marie-Pierre
Title:Development and evaluation of a core genome multilocus sequence typing scheme for Paenibacillus larvae, the deadly American foulbrood pathogen of honeybees
Series Name/Journal:Environmental Microbiology
Year of publishing :2021
Number of Pages:10
Publisher:WILEY
ISSN:1462-2912
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Microbiology (Microbiology in the medical area to be 30109)
URN:NBN:urn:nbn:se:slu:epsilon-p-111105
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-111105
Additional ID:
Type of IDID
DOI10.1111/1462-2920.15442
Web of Science (WoS)000624013200001
ID Code:25940
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Ecology
(S) > Dept. of Ecology
Deposited By: SLUpub Connector
Deposited On:25 Oct 2021 08:25
Metadata Last Modified:25 Oct 2021 08:31

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