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Listeria monocytogenes

strain diversity demonstrated by genotyping

Unnerstad, Helle (2001). Listeria monocytogenes. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae. Veterinaria, 1401-6257 ; 99
ISBN 91-576-5919-2
[Doctoral thesis]

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Abstract

The bacterium Listeria monocytogenes can cause the disease listeriosis in both humans and animals. For humans, the infection is mainly foodborne. Since the bacterium is able to grow even at refrigerator temperature, a food product may constitute a possible vehicle of infection although stored in cold environment. Meningoencephalitis and septicaemia are clinical manifestations of listeriosis. Infection in a pregnant woman may lead to abortion of the foetus or the birth of a seriously ill child. Risk groups for L. monocytogenes infection are immunocompromised persons, the elderly, pregnant women and infants. Different methods for visualising the genetic diversity among strains of L. monocytogenes were used in this thesis. L. monocytogenes can be established in food processing plants for long periods. Investigation of genetic diversity among strains isolated from a dairy during several years by pulsed-field gel electrophoresis (PFGE) showed indistinguishable restriction patterns. This indicated a common source for the L. monocytogenes contamination. The PFGE method was also used to investigate the diversity among strains isolated from faeces from clinically healthy cows. Polymerase chain reaction and restriction enzyme analysis (PCR-REA) of a segment of a virulence gene region could divide strains of serovar 1/2a into two groups. Strain diversity was also visualised by sequencing. Both conventional sequencing of 1500 bp of the virulence gene inlB and the sequencing-by-synthesis method pyrosequencing of two positions of this gene, grouped L. monocytogenes strains in a similar way. Strains of serovars 1/2a and 1/2c formed one group, strains of serovar 1/2b and 3b another and strains of serovar 4b were divided into two groups. Based on nucleotide polymorphisms in the inlB gene the strain diversity within serovar 4b was more distinct than within the other investigated serovar

Authors/Creators:Unnerstad, Helle
Title:Listeria monocytogenes
Subtitle:strain diversity demonstrated by genotyping
Year of publishing :January 2001
Volume:99
Number of Pages:36
Papers/manuscripts:
NumberReferences
ALLI. Unnerstad, H., Bannerman, E., Bille, J., Danielsson-Tham, M.-L., Waak, E. and Tham, W. 1996. Prolonged contamination of a dairy with Listeria monocytogenes. Netherlands Milk and Dairy Journal 50, 493-499. II. Unnerstad, H., Romell, A., Ericsson, H., Danielsson-Tham, M.-L. and Tham, W. 2000. Listeria monocytogenes in faeces from clinically healthy dairy cows in Sweden. Acta Veterinaria Scandinavica 41, 167-171. III. Unnerstad, H., Nilsson, I., Ericsson, H., Danielsson-Tham, M.-L., Bille, J., Bannerman, E. and Tham, W. 1999. Division of Listeria monocytogenes serovar 1/2a strains into two groups by PCR and restriction enzyme analysis. Applied and Environmental Microbiology 65, 2054-2056. IV. Ericsson, H., Unnerstad, H., Mattsson, J. G., Danielsson-Tham, M.-L. and Tham, W. 2000. Molecular grouping of Listeria monocytogenes based on the sequence of the inlB gene. Journal of Medical Microbiology 49, 73-80. V. Unnerstad, H., Ericsson, H., Alderborn, A., Tham, W., Danielsson-Tham, M.-L. and Mattsson, J. G. Pyrosequencing as a method for grouping of Listeria monocytogenes strains based on single nucleotide polymorphisms in the inlB gene. Submitte
Place of Publication:Uppsala
ISBN for printed version:91-576-5919-2
ISSN:1401-6257
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:Q Food science > Q53 Feed contamination and toxicology
Subjects:Not in use, please see Agris categories
Agrovoc terms:listeria monocytogenes, bacterioses, human diseases, foodborne diseases, disease transmission, diagnosis, genetic variation, electrophoresis, restriction enzymes, pcr, nucleotides
Keywords:Listeria monocytogenes, foodborne pathogen, contamination, genetic diversity, typing, pulsed-field gel electrophoresis, restriction pattern, nucleotide polymorphisms.
ID Code:26
Department:?? 7022 ??
Deposited By: Staff Epsilon
Deposited On:30 Apr 2002 00:00
Metadata Last Modified:02 Dec 2014 10:01

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