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Suspect and non-target screening of ovarian follicular fluid and serum - identification of anthropogenic chemicals and investigation of their association to fertility

Hallberg, Ida and Plassmann, Merle and Olovsson, Matts and Holte, Jan and Damdimopoulou, Pauliina and Sjunnesson, Ylva and Benskin, Jonathan P. and Persson, Sara (2021). Suspect and non-target screening of ovarian follicular fluid and serum - identification of anthropogenic chemicals and investigation of their association to fertility. Environmental Science: Processes and Impacts. 23 , 1578-1588
[Research article]

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Abstract

In this work, ultra-high performance liquid chromatography-high resolution (Orbitrap) mass spectrometry-based suspect and non-target screening was applied to follicular fluid (n = 161) and serum (n = 116) from women undergoing in vitro fertilization in order to identify substances that may be associated with decreased fertility. Detected features were prioritized for identification based on (i) hazard/exposure scores in a database of chemicals on the Swedish market and an in-house database on per- and polyfluoroalkyl substances (PFAS); (ii) enrichment in follicular fluid relative to serum; and (iii) association with treatment outcomes. Non-target screening detected 20 644 features in follicular fluid and 13 740 in serum. Two hundred and sixty-two features accumulated in follicular fluid (follicular fluid: serum ratio >20) and another 252 features were associated with embryo quality. Standards were used to confirm the identities of 21 compounds, including 11 PFAS. 6-Hydroxyindole was associated with lower embryo quality and 4-aminophenol was associated with higher embryo quality. Overall, we show the complexity of follicular fluid and the applicability of suspect and non-target screening for discovering both anthropogenic and endogenous substances, which may play a role in fertility in women.

Authors/Creators:Hallberg, Ida and Plassmann, Merle and Olovsson, Matts and Holte, Jan and Damdimopoulou, Pauliina and Sjunnesson, Ylva and Benskin, Jonathan P. and Persson, Sara
Title:Suspect and non-target screening of ovarian follicular fluid and serum - identification of anthropogenic chemicals and investigation of their association to fertility
Series Name/Journal:Environmental Science: Processes and Impacts
Year of publishing :2021
Volume:23
Page range:1578-1588
Number of Pages:11
Publisher:ROYAL SOC CHEMISTRY
ISSN:2050-7887
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 105 Earth and Related Environmental Sciences > Environmental Sciences (social aspects to be 507)
URN:NBN:urn:nbn:se:slu:epsilon-p-113872
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-113872
Additional ID:
Type of IDID
DOI10.1039/d1em00211b
Web of Science (WoS)000700538600001
ID Code:26000
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
Deposited By: SLUpub Connector
Deposited On:01 Nov 2021 12:25
Metadata Last Modified:01 Nov 2021 12:31

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