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Candidate regulators and target genes of drought stress in needles and roots of Norway spruce

Haas, Julia C. and Vergara, Alexander and Serrano, Alonso and Mishra, Sanatkumar and Hurry, Vaughan and Street, Nathaniel R. (2021). Candidate regulators and target genes of drought stress in needles and roots of Norway spruce. Tree Physiology. 41 , 1230-1246
[Research article]

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Abstract

Drought stress impacts seedling establishment, survival and whole-plant productivity. Molecular responses to drought stress have been most extensively studied in herbaceous species, mostly considering only aboveground tissues. Coniferous tree species dominate boreal forests, which are predicted to be exposed to more frequent and acute drought as a result of ongoing climate change. The associated impact at all stages of the forest tree life cycle is expected to have large-scale ecological and economic impacts. However, the molecular response to drought has not been comprehensively profiled for coniferous species. We assayed the physiological and transcriptional response of Picea abies (L.) H. Karst seedling needles and roots after exposure to mild and severe drought. Shoots and needles showed an extensive reversible plasticity for physiological measures indicative of drought-response mechanisms, including changes in stomatal conductance (g(s)), shoot water potential and abscisic acid (ABA). In both tissues, the most commonly observed expression profiles in response to drought were highly correlated with the ABA levels. Still, root and needle transcriptional responses contrasted, with extensive root-specific down-regulation of growth. Comparison between previously characterized Arabidopsis thaliana L. drought-response genes and P. abies revealed both conservation and divergence of transcriptional response to drought. In P. abies, transcription factors belonging to the ABA responsive element(ABRE) binding/ABRE binding factors ABA-dependent pathway had a more limited role. These results highlight the importance of profiling both above- and belowground tissues, and provide a comprehensive framework to advance the understanding of the drought response of P. abies. The results demonstrate that a short-term, severe drought induces severe physiological responses coupled to extensive transcriptome modulation and highlight the susceptibility of Norway spruce seedlings to such drought events.

Authors/Creators:Haas, Julia C. and Vergara, Alexander and Serrano, Alonso and Mishra, Sanatkumar and Hurry, Vaughan and Street, Nathaniel R.
Title:Candidate regulators and target genes of drought stress in needles and roots of Norway spruce
Series Name/Journal:Tree Physiology
Year of publishing :2021
Volume:41
Page range:1230-1246
Number of Pages:17
Publisher:OXFORD UNIV PRESS
ISSN:0829-318X
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution-Noncommercial 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Forest Science
Keywords:ABA, drought stress, gene expression, Norway spruce, Picea abies, RNA-Seq, transcriptome
URN:NBN:urn:nbn:se:slu:epsilon-p-114229
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-114229
Additional ID:
Type of IDID
DOI10.1093/treephys/tpaa178
Web of Science (WoS)000710988700010
ID Code:26064
Faculty:S - Faculty of Forest Sciences
Department:(S) > Dept. of Forest Genetics and Plant Physiology
Deposited By: SLUpub Connector
Deposited On:05 Nov 2021 10:25
Metadata Last Modified:05 Nov 2021 10:31

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