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Characterization of germline development and identification of genes associated with germline specification in pineapple

Zhao, Lihua and Liu, Liping and Liu, Yanhui and Dou, Xianying and Cai, Hanyang and Aslam, Mohammad and Hou, Zhimin and Jin, Xingyue and Li, Yi and Wang, Lulu and Zhao, Heming and Wang, Xiaomei and Sicard, Adrien and Qin, Yuan (2021). Characterization of germline development and identification of genes associated with germline specification in pineapple. Horticulture Research. 8 , 239
[Research article]

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Abstract

Understanding germline specification in plants could be advantageous for agricultural applications. In recent decades, substantial efforts have been made to understand germline specification in several plant species, including Arabidopsis, rice, and maize. However, our knowledge of germline specification in many agronomically important plant species remains obscure. Here, we characterized the female germline specification and subsequent female gametophyte development in pineapple using callose staining, cytological, and whole-mount immunolocalization analyses. We also determined the male germline specification and gametophyte developmental timeline and observed male meiotic behavior using chromosome spreading assays. Furthermore, we identified 229 genes that are preferentially expressed at the megaspore mother cell (MMC) stage during ovule development and 478 genes that are preferentially expressed at the pollen mother cell (PMC) stage of anther development using comparative transcriptomic analysis. The biological functions, associated regulatory pathways and expression patterns of these genes were also analyzed. Our study provides a convenient cytological reference for exploring pineapple germline development and a molecular basis for the future functional analysis of germline specification in related plant species.

Authors/Creators:Zhao, Lihua and Liu, Liping and Liu, Yanhui and Dou, Xianying and Cai, Hanyang and Aslam, Mohammad and Hou, Zhimin and Jin, Xingyue and Li, Yi and Wang, Lulu and Zhao, Heming and Wang, Xiaomei and Sicard, Adrien and Qin, Yuan
Title:Characterization of germline development and identification of genes associated with germline specification in pineapple
Series Name/Journal:Horticulture Research
Year of publishing :2021
Volume:8
Article number:239
Number of Pages:21
Publisher:NANJING AGRICULTURAL UNIV
ISSN:2052-7276
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Horticulture
URN:NBN:urn:nbn:se:slu:epsilon-p-114293
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-114293
Additional ID:
Type of IDID
DOI10.1038/s41438-021-00669-x
Web of Science (WoS)000713155100011
ID Code:26090
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231)
Deposited By: SLUpub Connector
Deposited On:11 Nov 2021 14:25
Metadata Last Modified:11 Nov 2021 14:31

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