Abstract

Rhodotorula toruloides is an oleaginous yeast with high biotechnological potential. In order to understand the molecular physiology of lipid synthesis in R. toruloides and to advance metabolic engineering, a high-resolution genome is required. We constructed a genome draft of R. toruloides CBS 14, using a hybrid assembly approach, consisting of short and long reads generated by Illumina and Nanopore sequencing, respectively. The genome draft consists of 23 contigs and 3 scaffolds, with a N50 length of 1,529,952 bp, thus largely representing chromosomal organization. The total size of the genome is 20,534,857 bp and the overall GC content is 61.83%. Transcriptomic data from different growth conditions was used to aid species-specific gene annotation. We annotated 9464 genes and identified 11,691 transcripts. Furthermore, we demonstrated the presence of a potential plasmid, an extrachromosomal circular structure of about 11 kb with a copy number about three times as high as the other chromosomes.

Keywords

Rhodotorula toruloides; de-novo hybrid assembly; Nanopore sequencing; Annotation

Published in

Genomics
2021, Volume: 113, number: 6, pages: 4022-4027
Publisher: ACADEMIC PRESS INC ELSEVIER SCIENCE

SLU Authors

• Martín Hernández, Giselle De La Caridad

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Müller, Bettina

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Chmielarz, Mikolaj

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Passoth, Volkmar

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Genetics


Publication identifier

DOI: https://doi.org/10.1016/j.ygeno.2021.10.006

Permanent link to this page (URI)

https://res.slu.se/id/publ/114296