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Sex without crossing over in the yeast Saccharomycodes ludwigii

Papaioannou, Ioannis A. and Dutreux, Fabien and Peltier, France A. and Maekawa, Hiromi and Delhomme, Nicolas and Bardhan, Amit and Friedrich, Anne and Schacherer, Joseph and Knop, Michael (2021). Sex without crossing over in the yeast Saccharomycodes ludwigii. Genome Biology. 22 , 303
[Research article]

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Abstract

Background Intermixing of genomes through meiotic reassortment and recombination of homologous chromosomes is a unifying theme of sexual reproduction in eukaryotic organisms and is considered crucial for their adaptive evolution. Previous studies of the budding yeast species Saccharomycodes ludwigii suggested that meiotic crossing over might be absent from its sexual life cycle, which is predominated by fertilization within the meiotic tetrad. Results We demonstrate that recombination is extremely suppressed during meiosis in Sd. ludwigii. DNA double-strand break formation by the conserved transesterase Spo11, processing and repair involving interhomolog interactions are required for normal meiosis but do not lead to crossing over. Although the species has retained an intact meiotic gene repertoire, genetic and population analyses suggest the exceptionally rare occurrence of meiotic crossovers in its genome. A strong AT bias of spontaneous mutations and the absence of recombination are likely responsible for its unusually low genomic GC level. Conclusions Sd. ludwigii has followed a unique evolutionary trajectory that possibly derives fitness benefits from the combination of frequent mating between products of the same meiotic event with the extreme suppression of meiotic recombination. This life style ensures preservation of heterozygosity throughout its genome and may enable the species to adapt to its environment and survive with only minimal levels of rare meiotic recombination. We propose Sd. ludwigii as an excellent natural forum for the study of genome evolution and recombination rates.

Authors/Creators:Papaioannou, Ioannis A. and Dutreux, Fabien and Peltier, France A. and Maekawa, Hiromi and Delhomme, Nicolas and Bardhan, Amit and Friedrich, Anne and Schacherer, Joseph and Knop, Michael
Title:Sex without crossing over in the yeast Saccharomycodes ludwigii
Series Name/Journal:Genome Biology
Year of publishing :2021
Volume:22
Article number:303
Number of Pages:28
Publisher:BMC
ISSN:1474-760X
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Microbiology (Microbiology in the medical area to be 30109)
Keywords:Achiasmate meiosis, Automixis, Crossing over, Intratetrad mating, Meiotic recombination, Linkage disequilibrium, Mutation accumulation, Mutation rate, Saccharomycodes ludwigii
URN:NBN:urn:nbn:se:slu:epsilon-p-114341
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-114341
Additional ID:
Type of IDID
DOI10.1186/s13059-021-02521-w
Web of Science (WoS)000714355900001
ID Code:26121
Faculty:S - Faculty of Forest Sciences
Department:(S) > Dept. of Forest Genetics and Plant Physiology
Deposited By: SLUpub Connector
Deposited On:17 Nov 2021 14:25
Metadata Last Modified:17 Nov 2021 14:31

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