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Transcriptome Analysis of Potato Infected with the Necrotrophic Pathogen Alternaria solani

Brouwer, Sophie and Brus-Szkalej, Maja and Saripella, Ganapathi Varma and Liang, Dong and Liljeroth, Erland and Grenville-Briggs Didymus, Laura (2021). Transcriptome Analysis of Potato Infected with the Necrotrophic Pathogen Alternaria solani. Plants. 10 , 2212
[Research article]

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Abstract

Potato early blight is caused by the necrotrophic fungus Alternaria solani and can result in yield losses of up to 50% if left uncontrolled. At present, the disease is controlled by chemical fungicides, yet rapid development of fungicide resistance renders current control strategies unsustainable. On top of that, a lack of understanding of potato defences and the quantitative nature of resistance mechanisms against early blight hinders the development of more sustainable control methods. Necrotrophic pathogens, compared to biotrophs, pose an extra challenge to the plant, since common defence strategies to biotic stresses such as the hypersensitive response and programmed cell death are often beneficial for necrotrophs. With the aim of unravelling plant responses to both the early infection stages (i.e., before necrosis), such as appressorium formation and penetration, as well as to later responses to the onset of necrosis, we present here a transcriptome analysis of potato interactions with A. solani from 1 h after inoculation when the conidia have just commenced germination, to 48 h post inoculation when multiple cell necrosis has begun. Potato transcripts with putative functions related to biotic stress tolerance and defence against pathogens were upregulated, including a putative Nudix hydrolase that may play a role in defence against oxidative stress. A. solani transcripts encoding putative pathogenicity factors, such as cell wall degrading enzymes and metabolic processes that may be important for infection. We therefore identified the differential expression of several potato and A. solani transcripts that present a group of valuable candidates for further studies into their roles in immunity or disease development.

Authors/Creators:Brouwer, Sophie and Brus-Szkalej, Maja and Saripella, Ganapathi Varma and Liang, Dong and Liljeroth, Erland and Grenville-Briggs Didymus, Laura
Title:Transcriptome Analysis of Potato Infected with the Necrotrophic Pathogen Alternaria solani
Series Name/Journal:Plants
Year of publishing :2021
Volume:10
Article number:2212
Number of Pages:18
Publisher:MDPI
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science
Keywords:early blight, RNAseq, necrotroph, Solanum tuberosum, plant-pathogen interaction
URN:NBN:urn:nbn:se:slu:epsilon-p-114361
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-114361
Additional ID:
Type of IDID
DOI10.3390/plants10102212
Web of Science (WoS)000714059700001
ID Code:26134
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Protection Biology
(LTJ, LTV) > Department of Plant Breeding (from 130101)
Deposited By: SLUpub Connector
Deposited On:18 Nov 2021 15:26
Metadata Last Modified:18 Nov 2021 15:31

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