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A Chromosome-Level Assembly of Blunt Snout Bream (Megalobrama amblycephala) Genome Reveals an Expansion of Olfactory Receptor Genes in Freshwater Fish

Liu, Han and Chen, Chunhai and Lv, Maolin and Liu, Ning and Hu, Yafei and Zhang, Hailin and Enbody, Erik D. and Gao, Zexia and Andersson, Leif and Wang, Weimin (2021). A Chromosome-Level Assembly of Blunt Snout Bream (Megalobrama amblycephala) Genome Reveals an Expansion of Olfactory Receptor Genes in Freshwater Fish. Molecular Biology and Evolution. 38 , 4238-4251
[Research article]

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Abstract

The number of olfactory receptor genes (ORs), which are responsible for detecting diverse odor molecules varies extensively among mammals as a result of frequent gene gains and losses that contribute to olfactory specialization. However, how OR expansions/contractions in fish are influenced by habitat and feeding habit and which OR subfamilies are important in each ecological niche is unknown. Here, we report a major OR expansion in a freshwater herbivorous fish, Megalobrama amblycephala, using a highly contiguous, chromosome-level assembly. We evaluate the possible contribution of OR expansion to habitat and feeding specialization by comparing the OR repertoire in 28 phylogenetically and ecologically diverse teleosts. In total, we analyzed > 4,000 ORs including 3,253 intact, 122 truncated, and 913 pseudogenes. The number of intact ORs is highly variable ranging from 20 to 279. We estimate that the most recent common ancestor of Osteichthyes had 62 intact ORs, which declined in most lineages except the freshwater Otophysa clade that has a substantial expansion in subfamily beta and epsilon ORs. Across teleosts, we found a strong association between duplications of beta and epsilon ORs and freshwater habitat. Nearly, all ORs were expressed in the olfactory epithelium (OE) in three tested fish species. Specifically, all the expanded beta and epsilon ORs were highly expressed in OE of M. amblycephala. Together, we provide molecular and functional evidence for how OR repertoires in fish have undergone gain and loss with respect to ecological factors and highlight the role of beta and epsilon OR in freshwater adaptation.

Authors/Creators:Liu, Han and Chen, Chunhai and Lv, Maolin and Liu, Ning and Hu, Yafei and Zhang, Hailin and Enbody, Erik D. and Gao, Zexia and Andersson, Leif and Wang, Weimin
Title:A Chromosome-Level Assembly of Blunt Snout Bream (Megalobrama amblycephala) Genome Reveals an Expansion of Olfactory Receptor Genes in Freshwater Fish
Series Name/Journal:Molecular Biology and Evolution
Year of publishing :2021
Volume:38
Page range:4238-4251
Number of Pages:14
Publisher:OXFORD UNIV PRESS
ISSN:0737-4038
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Evolutionary Biology
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Genetics (medical genetics to be 30107 and agricultural genetics to be 40402)
Keywords:Cyprinid fish, comparative genomics, olfactory receptor, evolutionary dynamics, ecological adaptation
URN:NBN:urn:nbn:se:slu:epsilon-p-114390
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-114390
Additional ID:
Type of IDID
DOI10.1093/molbev/msab152
Web of Science (WoS)000715359700011
ID Code:26181
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Animal Breeding and Genetics
Deposited By: SLUpub Connector
Deposited On:22 Nov 2021 08:25
Metadata Last Modified:22 Nov 2021 08:31

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