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Secretory mouse quiescin sulfhydryl oxidase 1 aggregates defected human and mouse spermatozoa in vitro and in vivo

Wang, Tse-En and Yeh, Ling-Yu and Lee, Robert Kuo-Kuang and Lu, Chung-Hao and Yang, Tsung-Hsien and Kuo, Yu-Wen and Joshi, Radhika and Tsai, Pei-Shiue and Li, Sheng-Hsiang (2021). Secretory mouse quiescin sulfhydryl oxidase 1 aggregates defected human and mouse spermatozoa in vitro and in vivo. iScience. 24 , 103167
[Research article]

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Abstract

A flavin-dependent enzyme quiescin Q6 sulfhydryl oxidase 1 (QSOX1) catalyzes the oxidation of thiol groups into disulfide bonds. QSOX1 is prominently expressed in the seminal plasma. However, its role in male reproduction is elusive. Here, we purified the secreted form of QSOX1, i.e., QSOX1c, from mouse seminal vesicle secretions and revealed for the first time its function involved in sperm physiology. Exogenous addition of QSOX1c time-dependently promoted the in vitro aggregation of thiol-rich, oxidative stressed, and apoptotic mouse and human sperm cells. Also, in vivo aggregated sperm cells collected from mouse uterine and human ejaculates also showed high levels of QSOX1c, intracellular reactive oxygen species, annexin V, and free thiols. In summary, our studies demonstrated that QSOX1c could agglutinate spermatozoa susceptible to free radical attack and apoptosis. This characteristic may provide an opportunity to separate defective sperm cells and improve sperm quality before artificial insemination in humans and animals.

Authors/Creators:Wang, Tse-En and Yeh, Ling-Yu and Lee, Robert Kuo-Kuang and Lu, Chung-Hao and Yang, Tsung-Hsien and Kuo, Yu-Wen and Joshi, Radhika and Tsai, Pei-Shiue and Li, Sheng-Hsiang
Title:Secretory mouse quiescin sulfhydryl oxidase 1 aggregates defected human and mouse spermatozoa in vitro and in vivo
Series Name/Journal:iScience
Year of publishing :2021
Volume:24
Article number:103167
Number of Pages:19
Publisher:CELL PRESS
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution-Noncommercial-No Derivative Works 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
URN:NBN:urn:nbn:se:slu:epsilon-p-114386
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-114386
Additional ID:
Type of IDID
DOI10.1016/j.isci.2021.103167
Web of Science (WoS)000713820000004
ID Code:26189
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
Deposited By: SLUpub Connector
Deposited On:22 Nov 2021 11:25
Metadata Last Modified:22 Nov 2021 11:31

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