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Physiological properties, composition and structural profiling of porcine gastrointestinal mucus

Barmpatsalou, Vicky and Dubbelboer, Ilse R. and Rodler, Agnes and Jacobson, Magdalena and Karlsson, Eva and Pedersen, Betty Lomstein and Bergstrom, Christel A. S. (2021). Physiological properties, composition and structural profiling of porcine gastrointestinal mucus. European Journal of Pharmaceutics and Biopharmaceutics. 169 , 156-167
[Research article]

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Abstract

The gastrointestinal mucus is a hydrogel that lines the luminal side of the gastrointestinal epithelium, offering barrier protection from pathogens and lubrication of the intraluminal contents. These barrier properties likewise affect nutrients and drugs that need to penetrate the mucus to reach the epithelium prior to absorption. In order to assess the potential impact of the mucus on drug absorption, we need information about the nature of the gastrointestinal mucus. Today, most of the relevant available literature is mainly derived from rodent studies. In this work, we used a larger animal species, the pig model, to characterize the mucus throughout the length of the gastrointestinal tract. This is the first report of the physiological properties (physical appearance, pH and water content), composition (protein, lipid and metabolite content) and structural profiling (rheology and gel network) of the porcine gastrointestinal mucus. These findings allow for direct comparisons between the characteristics of mucus from various segments and can be further utilized to improve our understanding of the role of the mucus on region dependent drug absorption. Additionally, the present work is expected to contribute to the assessment of the porcine model as a preclinical species in the drug development process.

Authors/Creators:Barmpatsalou, Vicky and Dubbelboer, Ilse R. and Rodler, Agnes and Jacobson, Magdalena and Karlsson, Eva and Pedersen, Betty Lomstein and Bergstrom, Christel A. S.
Title:Physiological properties, composition and structural profiling of porcine gastrointestinal mucus
Series Name/Journal:European Journal of Pharmaceutics and Biopharmaceutics
Year of publishing :2021
Volume:169
Page range:156-167
Number of Pages:12
Publisher:ELSEVIER
ISSN:0939-6411
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution-Noncommercial-No Derivative Works 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
Keywords:Porcine, Gastrointestinal, Mucus, Composition, Structure, Proteomics, Lipidomics, Rheology, Cryo-SEM
URN:NBN:urn:nbn:se:slu:epsilon-p-114433
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-114433
Additional ID:
Type of IDID
DOI10.1016/j.ejpb.2021.10.008
Web of Science (WoS)000718177400004
ID Code:26210
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
Deposited By: SLUpub Connector
Deposited On:25 Nov 2021 13:25
Metadata Last Modified:25 Nov 2021 13:31

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