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Use of bacterial strains antagonistic to Escherichia coli for biocontrol of spinach: A field trial

Uhlig, E. and Kjellstrom, A. and Nurminen, N. and Olsson, C. and Oscarsson, E. and Canaviri-Paz, P. and Mogren, Lars and Alsanius, Beatrix and Molin, G. and Hakansson, A. (2021). Use of bacterial strains antagonistic to Escherichia coli for biocontrol of spinach: A field trial. Innovative Food Science and Emerging Technologies. 74 , 102862
[Research article]

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Abstract

To counteract global food safety hazards related to raw consumption of ready-to-eat leafy vegetables, a method to improve bacterial status using antagonistic bacteria was studied under field conditions. This is the first study to identify potential Escherichia coli antagonists from the native microbiota on leafy green vegetables and evaluate their effect in an industrial field production setting. Bacterial strains were isolated from different types of leafy green vegetables and selected upon their effect against E. coli in vitro, and out of 295 tested bacterial strains, 37 showed an antagonistic effect. Four of those antagonistic strains were coated in separate treatments onto spinach seeds and planted in the field. Both seeds and plants were analyzed by Illumina MiSeq next generation sequencing (NGS), and it was seen that the microbiota of the plants contained lower relative abundance of plant and human pathogenic genera. Higher beta-diversity was observed for the samples treated with Bacillus coagulans LMG P-32205 and B. coagulans LMG P-32206 compared to control, indicating that those strains have induced substantial changes in the native microbiota of the leaves. A reduction of Escherichia-Shigella was seen for two of the isolates (Pseudomonas cedrina LMG P-32207 and Pseudomonas punonenis LMG P-32204) as the seeds developed into plants. Seeds inoculated with two of the strains (B. coagulans LMG P-32205 and B. coagulans LMG P-32206) had increased levels of Lactobacillaceae, and treatment with B. coagulans LMG P-32206 resulted in lower levels of Pantoea (from 31.4 to 12.2%). These results encourage the usage of bacterial antagonists as part of a global solution to reduce the risk of human pathogens on leafy green vegetables.

Authors/Creators:Uhlig, E. and Kjellstrom, A. and Nurminen, N. and Olsson, C. and Oscarsson, E. and Canaviri-Paz, P. and Mogren, Lars and Alsanius, Beatrix and Molin, G. and Hakansson, A.
Title:Use of bacterial strains antagonistic to Escherichia coli for biocontrol of spinach: A field trial
Series Name/Journal:Innovative Food Science and Emerging Technologies
Year of publishing :2021
Volume:74
Article number:102862
Number of Pages:10
Publisher:ELSEVIER SCI LTD
Associated Programs and Other Stakeholders:Z - SLU - Library > Odla mera
ISSN:1466-8564
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Food Science
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Pathobiology
Keywords:Leafy-green vegetables, Escherichia coli, Native microbiota, Biological control
URN:NBN:urn:nbn:se:slu:epsilon-p-114437
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-114437
Additional ID:
Type of IDID
DOI10.1016/j.ifset.2021.102862
Web of Science (WoS)000716882500004
ID Code:26213
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Biosystems and Technology (from 130101)
Deposited By: SLUpub Connector
Deposited On:26 Nov 2021 07:25
Metadata Last Modified:27 Nov 2021 05:01

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