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Artificial spider silk supports and guides neurite extension in vitro

Hansson, Magnus L. and Chatterjee, Urmimala and Francis, Juanita and Arndt, Tina and Broman, Christian and Johansson, Jan and Skold, Mattias K. and Rising, Anna (2021). Artificial spider silk supports and guides neurite extension in vitro. FASEB Journal. 35 , e21896
[Research article]

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Abstract

Surgical intervention with the use of autografts is considered the gold standard to treat peripheral nerve injuries. However, a biomaterial that supports and guides nerve growth would be an attractive alternative to overcome problems with limited availability, morbidity at the site of harvest, and nerve mismatches related to autografts. Native spider silk is a promising material for construction of nerve guidance conduit (NGC), as it enables regeneration of cm-long nerve injuries in sheep, but regulatory requirements for medical devices demand synthetic materials. Here, we use a recombinant spider silk protein (NT2RepCT) and a functionalized variant carrying a peptide derived from vitronectin (VN-NT2RepCT) as substrates for nerve growth support and neurite extension, using a dorsal root ganglion cell line, ND7/23. Two-dimensional coatings were benchmarked against poly-d-lysine and recombinant laminins. Both spider silk coatings performed as the control substrates with regards to proliferation, survival, and neurite growth. Furthermore, NT2RepCT and VN-NT2RepCT spun into continuous fibers in a biomimetic spinning set-up support cell survival, neurite growth, and guidance to an even larger extent than native spider silk. Thus, artificial spider silk is a promising biomaterial for development of NGCs.

Authors/Creators:Hansson, Magnus L. and Chatterjee, Urmimala and Francis, Juanita and Arndt, Tina and Broman, Christian and Johansson, Jan and Skold, Mattias K. and Rising, Anna
Title:Artificial spider silk supports and guides neurite extension in vitro
Series Name/Journal:FASEB Journal
Year of publishing :2021
Volume:35
Article number:e21896
Number of Pages:14
Publisher:WILEY
ISSN:0892-6638
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution-Noncommercial-No Derivative Works 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 2 Engineering and Technology > 206 Medical Engineering > Medical Materials
Keywords:biomaterial, nerve guidance conduit, peripheral nerve, recombinant spider silk
URN:NBN:urn:nbn:se:slu:epsilon-p-114470
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-114470
Additional ID:
Type of IDID
DOI10.1096/fj.202100916R
Web of Science (WoS)000712009200019
ID Code:26225
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Anatomy, Physiology and Biochemistry
Deposited By: SLUpub Connector
Deposited On:29 Nov 2021 10:25
Metadata Last Modified:29 Nov 2021 10:31

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