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Identification of bull semen microbiome by 16S sequencing and possible relationships with fertility

Cojkic, Aleksandar and Niazi, Adnan and Guo, Yongzhi and Hallap, Triin and Padrik, Peeter and Morrell, Jane (2021). Identification of bull semen microbiome by 16S sequencing and possible relationships with fertility. Microorganisms. 9 , 2431
[Research article]

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Abstract

Reports on the use of 16S sequencing for the identification of bacteria in healthy animals are lacking. Bacterial contamination of bull semen can have a negative effect on the sperm quality. The aims of this study were threefold: to identify bacteria in the semen of healthy bulls using 16S sequencing; to investigate the differences in the bacterial community between individual bulls; and to establish if there was a relationship between the bacteria isolated and bull fertility. Semen from 18 bulls of known fertility was used for the DNA extraction and 16S sequencing; 107 bacterial genera were identified. The differences in the amplicon sequence variants (ASVs) and the numbers of genera between bulls were noted. Negative correlations (p < 0.05) between several bacterial genera with Curvibacter, Rikenellaceae RC9-gut-group and Dyella spp. were seen. Other negatively correlated bacteria were Cutibacterium, Ruminococcaceae UCG-005, Ruminococcaceae UCG-010 and Staphylococcus, all within the top 20 genera. Two genera, W5053 and Lawsonella, were enriched in bulls of low fertility; this is the first time that these bacteria have been reported in bull semen samples. The majority of the bacteria were environmental organisms or were species originating from the mucous membranes of animals and humans. The results of this study indicate that differences in the seminal microbiota of healthy bulls occur and might be correlated with fertility.

Authors/Creators:Cojkic, Aleksandar and Niazi, Adnan and Guo, Yongzhi and Hallap, Triin and Padrik, Peeter and Morrell, Jane
Title:Identification of bull semen microbiome by 16S sequencing and possible relationships with fertility
Series Name/Journal:Microorganisms
Year of publishing :2021
Volume:9
Article number:2431
Number of Pages:12
ISSN:2076-2607
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 402 Animal and Dairy Science > Animal and Dairy Science.
Keywords:bull semen, bacteria, 16S sequencing, sperm quality, fertility
URN:NBN:urn:nbn:se:slu:epsilon-p-114498
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-114498
Additional ID:
Type of IDID
DOI10.3390/microorganisms9122431
ID Code:26264
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
(VH) > Dept. of Animal Breeding and Genetics
Deposited By: SLUpub Connector
Deposited On:02 Dec 2021 13:25
Metadata Last Modified:02 Dec 2021 13:31

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