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In vitro Selection of Synbiotics and in vivo Investigation of Growth Indices, Reproduction Performance, Survival, and Ovarian Cyp19 alpha Gene Expression in Zebrafish Danio rerio

Zakariaee, Hamideh and Sudagar, Mohammad and Hosseini, Seyede Sedighe and Paknejad, Hamed and Baruah, Kartik (2021). In vitro Selection of Synbiotics and in vivo Investigation of Growth Indices, Reproduction Performance, Survival, and Ovarian Cyp19 alpha Gene Expression in Zebrafish Danio rerio. Frontiers in Microbiology. 12 , 758758
[Research article]

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Abstract

In this study, we tested the compatibility of two extracts from the plant Jerusalem artichokes and button mushrooms with two different Lactobacillus probiotics (Lactobacillus acidophilus; La and Lactobacillus delbrueckii subsp. Bulgaricus; Lb) to develop a synbiotic formulation to improve the growth, survival, and reproductive performances of farmed fishes. Initially, we employed in vitro approach to monitor the growth of the probiotic lactobacilli in the presence of the different doses of the plant-based prebiotics, with the aim of selecting interesting combination(s) for further verification under in vivo conditions using zebrafish as a model. Results from the in vitro screening assay in the broth showed that both the probiotic species showed a preference for 50% mushroom extract as a source of prebiotic. A synbiotic formulation, developed with the selected combination of L. acidophilus, L. bulgaricus, and 50% mushroom extract, showed a positive influence on the growth and reproductive performances of the zebrafish. Our findings also imply that the improvement in the reproductive indices was associated with the upregulation of a cyp19a gene. Overall results suggest that a combination of L. acidophilus, L. bulgaricus, and mushroom extract can be considered as a potential synbiotic for the successful production of aquaculture species.

Authors/Creators:Zakariaee, Hamideh and Sudagar, Mohammad and Hosseini, Seyede Sedighe and Paknejad, Hamed and Baruah, Kartik
Title:In vitro Selection of Synbiotics and in vivo Investigation of Growth Indices, Reproduction Performance, Survival, and Ovarian Cyp19 alpha Gene Expression in Zebrafish Danio rerio
Series Name/Journal:Frontiers in Microbiology
Year of publishing :2021
Volume:12
Article number:758758
Number of Pages:16
Publisher:FRONTIERS MEDIA SA
ISSN:1664-302X
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Medical Bioscience
Keywords:prebiotics, probiotics, zebrafish, mushroom, artichoke, reproduction, cyp19a gene
URN:NBN:urn:nbn:se:slu:epsilon-p-114614
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-114614
Additional ID:
Type of IDID
DOI10.3389/fmicb.2021.758758
Web of Science (WoS)000717044800001
ID Code:26288
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Animal Nutrition and Management
Deposited By: SLUpub Connector
Deposited On:09 Dec 2021 09:54
Metadata Last Modified:09 Dec 2021 10:01

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