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Jasmonate inhibits adventitious root initiation through repression of CKX1 and activation of RAP2.6L transcription factor in Arabidopsis

Dob, Asma and Lakehal, Abdellah and Novak, Ondrej and Bellini, Catherine (2021). Jasmonate inhibits adventitious root initiation through repression of CKX1 and activation of RAP2.6L transcription factor in Arabidopsis. Journal of Experimental Botany. 72 , 7107-7118
[Research article]

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Abstract

Adventitious rooting is a de novo organogenesis process that enables plants to propagate clonally and cope with environmental stresses. Adventitious root initiation (ARI) is controlled by interconnected transcriptional and hormonal networks, but there is little knowledge of the genetic and molecular programs orchestrating these networks. Thus, we have applied genome-wide transcriptome profiling to elucidate the transcriptional reprogramming events preceding ARI. These reprogramming events are associated with the down-regulation of cytokinin (CK) signaling and response genes, which could be triggers for ARI. Interestingly, we found that CK free base (iP, tZ, cZ, and DHZ) content declined during ARI, due to down-regulation of de novo CK biosynthesis and up-regulation of CK inactivation pathways. We also found that MYC2-dependent jasmonate (JA) signaling inhibits ARI by down-regulating the expression of the CYTOKININ OXIDASE/DEHYDROGENASE1 (CKX1) gene. We also demonstrated that JA and CK synergistically activate expression of the transcription factor RELATED to APETALA2.6 LIKE (RAP2.6L), and constitutive expression of this transcription factor strongly inhibits ARI. Collectively, our findings reveal that previously unknown genetic interactions between JA and CK play key roles in ARI.

Authors/Creators:Dob, Asma and Lakehal, Abdellah and Novak, Ondrej and Bellini, Catherine
Title:Jasmonate inhibits adventitious root initiation through repression of CKX1 and activation of RAP2.6L transcription factor in Arabidopsis
Series Name/Journal:Journal of Experimental Botany
Year of publishing :2021
Volume:72
Page range:7107-7118
Number of Pages:12
Publisher:OXFORD UNIV PRESS
ISSN:0022-0957
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Botany
Keywords:Adventitious roots, Arabidopsis, CKX1, cytokinins, jasmonate, light, MYC2, RAP2.6L, vegetative propagation
URN:NBN:urn:nbn:se:slu:epsilon-p-114589
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-114589
Additional ID:
Type of IDID
DOI10.1093/jxb/erab358
Web of Science (WoS)000715558300024
ID Code:26296
Faculty:S - Faculty of Forest Sciences
Department:(S) > Dept. of Forest Genetics and Plant Physiology
Deposited By: SLUpub Connector
Deposited On:10 Dec 2021 09:25
Metadata Last Modified:10 Dec 2021 09:31

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