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Green Chemistry Production of Codlemone, the Sex Pheromone of the Codling Moth (Cydia pomonella), by Metabolic Engineering of the Oilseed Crop Camelina (Camelina sativa)

Xia, Yi-Han and Wang, Hong-Lei and Ding, Bao-Jian and Svensson, Glenn P. and Jarl-Sunesson, Carin and Cahoon, Edgar B. and Hofvander, Per and Lofstedt, Christer (2021). Green Chemistry Production of Codlemone, the Sex Pheromone of the Codling Moth (Cydia pomonella), by Metabolic Engineering of the Oilseed Crop Camelina (Camelina sativa). Journal of Chemical Ecology. 47 , 950-967
[Research article]

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Abstract

Synthetic pheromones have been used for pest control over several decades. The conventional synthesis of di-unsaturated pheromone compounds is usually complex and costly. Camelina (Camelina sativa) has emerged as an ideal, non-food biotech oilseed platform for production of oils with modified fatty acid compositions. We used Camelina as a plant factory to produce mono- and di-unsaturated C-12 chain length moth sex pheromone precursors, (E)-9-dodecenoic acid and (E,E)-8,10-dodecadienoic acid, by introducing a fatty acyl-ACP thioesterase FatB gene UcTE from California bay laurel (Umbellularia californica) and a bifunctional increment 9 desaturase gene Cpo_CPRQ from the codling moth, Cydia pomonella. Different transgene combinations were investigated for increasing pheromone precursor yield. The most productive Camelina line was engineered with a vector that contained one copy of UcTE and the viral suppressor protein encoding P19 transgenes and three copies of Cpo_CPRQ transgene. The T-2 generation of this line produced 9.4% of (E)-9-dodecenoic acid and 5.5% of (E,E)-8,10-dodecadienoic acid of the total fatty acids, and seeds were selected to advance top-performing lines to homozygosity. In the T-4 generation, production levels of (E)-9-dodecenoic acid and (E,E)-8,10-dodecadienoic acid remained stable. The diene acid together with other seed fatty acids were converted into corresponding alcohols, and the bioactivity of the plant-derived codlemone was confirmed by GC-EAD and a flight tunnel assay. Trapping in orchards and home gardens confirmed significant and specific attraction of C. pomonella males to the plant-derived codlemone.

Authors/Creators:Xia, Yi-Han and Wang, Hong-Lei and Ding, Bao-Jian and Svensson, Glenn P. and Jarl-Sunesson, Carin and Cahoon, Edgar B. and Hofvander, Per and Lofstedt, Christer
Title:Green Chemistry Production of Codlemone, the Sex Pheromone of the Codling Moth (Cydia pomonella), by Metabolic Engineering of the Oilseed Crop Camelina (Camelina sativa)
Series Name/Journal:Journal of Chemical Ecology
Year of publishing :2021
Volume:47
Page range:950-967
Number of Pages:18
Publisher:SPRINGER
ISSN:0098-0331
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science
Keywords:Conjugated double bonds, Plant factory, Acyl-ACP thioesterase, increment 9 desaturase, Multi-gene copies, P19, Agrobacterium-based floral-dip transformation, Bioassay
URN:NBN:urn:nbn:se:slu:epsilon-p-114458
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-114458
Additional ID:
Type of IDID
DOI10.1007/s10886-021-01316-4
Web of Science (WoS)000717435700001
ID Code:26304
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Breeding (from 130101)
Deposited By: SLUpub Connector
Deposited On:13 Dec 2021 15:25
Metadata Last Modified:13 Dec 2021 15:31

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