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Leaf Apoplast of Field-Grown Potato Analyzed by Quantitative Proteomics and Activity-Based Protein Profiling

Abreha, Kibrom Berhe and Alexandersson, Erik and Resjö, Svante and Lankinen, Åsa and Sueldo, Daniela and Kaschani, Farnusch and Kaiser, Markus and van der Hoorn, Renier A. L. and Levander, Fredrik and Andreasson, Erik (2021). Leaf Apoplast of Field-Grown Potato Analyzed by Quantitative Proteomics and Activity-Based Protein Profiling. International Journal of Molecular Sciences. 22 , 12033
[Research article]

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Abstract

Multiple biotic and abiotic stresses challenge plants growing in agricultural fields. Most molecular studies have aimed to understand plant responses to challenges under controlled conditions. However, studies on field-grown plants are scarce, limiting application of the findings in agricultural conditions. In this study, we investigated the composition of apoplastic proteomes of potato cultivar Bintje grown under field conditions, i.e., two field sites in June-August across two years and fungicide treated and untreated, using quantitative proteomics, as well as its activity using activity-based protein profiling (ABPP). Samples were clustered and some proteins showed significant intensity and activity differences, based on their field site and sampling time (June-August), indicating differential regulation of certain proteins in response to environmental or developmental factors. Peroxidases, class II chitinases, pectinesterases, and osmotins were among the proteins more abundant later in the growing season (July-August) as compared to early in the season (June). We did not detect significant differences between fungicide Shirlan treated and untreated field samples in two growing seasons. Using ABPP, we showed differential activity of serine hydrolases and beta-glycosidases under greenhouse and field conditions and across a growing season. Furthermore, the activity of serine hydrolases and beta-glycosidases, including proteins related to biotic stress tolerance, decreased as the season progressed. The generated proteomics data would facilitate further studies aiming at understanding mechanisms of molecular plant physiology in agricultural fields and help applying effective strategies to mitigate biotic and abiotic stresses.

Authors/Creators:Abreha, Kibrom Berhe and Alexandersson, Erik and Resjö, Svante and Lankinen, Åsa and Sueldo, Daniela and Kaschani, Farnusch and Kaiser, Markus and van der Hoorn, Renier A. L. and Levander, Fredrik and Andreasson, Erik
Title:Leaf Apoplast of Field-Grown Potato Analyzed by Quantitative Proteomics and Activity-Based Protein Profiling
Series Name/Journal:International Journal of Molecular Sciences
Year of publishing :2021
Volume:22
Article number:12033
Number of Pages:17
Publisher:MDPI
ISSN:1661-6596
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Botany
Keywords:ABPP, apoplast, proteomics, serine hydrolases, beta-glycosidases, potato, field-omics
URN:NBN:urn:nbn:se:slu:epsilon-p-114711
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-114711
Additional ID:
Type of IDID
DOI10.3390/ijms222112033
Web of Science (WoS)000722344300001
ID Code:26312
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Protection Biology
Deposited By: SLUpub Connector
Deposited On:15 Dec 2021 15:26
Metadata Last Modified:15 Dec 2021 15:31

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