Abreha, Kibrom Berhe and Alexandersson, Erik and Resjö, Svante and Lankinen, Åsa and Sueldo, Daniela and Kaschani, Farnusch and Kaiser, Markus and van der Hoorn, Renier A. L. and Levander, Fredrik and Andreasson, Erik
(2021).
Leaf Apoplast of Field-Grown Potato Analyzed by Quantitative Proteomics and Activity-Based Protein Profiling.
International Journal of Molecular Sciences. 22
, 12033
[Research article]
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Abstract
Multiple biotic and abiotic stresses challenge plants growing in agricultural fields. Most molecular studies have aimed to understand plant responses to challenges under controlled conditions. However, studies on field-grown plants are scarce, limiting application of the findings in agricultural conditions. In this study, we investigated the composition of apoplastic proteomes of potato cultivar Bintje grown under field conditions, i.e., two field sites in June-August across two years and fungicide treated and untreated, using quantitative proteomics, as well as its activity using activity-based protein profiling (ABPP). Samples were clustered and some proteins showed significant intensity and activity differences, based on their field site and sampling time (June-August), indicating differential regulation of certain proteins in response to environmental or developmental factors. Peroxidases, class II chitinases, pectinesterases, and osmotins were among the proteins more abundant later in the growing season (July-August) as compared to early in the season (June). We did not detect significant differences between fungicide Shirlan treated and untreated field samples in two growing seasons. Using ABPP, we showed differential activity of serine hydrolases and beta-glycosidases under greenhouse and field conditions and across a growing season. Furthermore, the activity of serine hydrolases and beta-glycosidases, including proteins related to biotic stress tolerance, decreased as the season progressed. The generated proteomics data would facilitate further studies aiming at understanding mechanisms of molecular plant physiology in agricultural fields and help applying effective strategies to mitigate biotic and abiotic stresses.
Authors/Creators: | Abreha, Kibrom Berhe and Alexandersson, Erik and Resjö, Svante and Lankinen, Åsa and Sueldo, Daniela and Kaschani, Farnusch and Kaiser, Markus and van der Hoorn, Renier A. L. and Levander, Fredrik and Andreasson, Erik | ||||||
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Title: | Leaf Apoplast of Field-Grown Potato Analyzed by Quantitative Proteomics and Activity-Based Protein Profiling | ||||||
Series Name/Journal: | International Journal of Molecular Sciences | ||||||
Year of publishing : | 2021 | ||||||
Volume: | 22 | ||||||
Article number: | 12033 | ||||||
Number of Pages: | 17 | ||||||
Publisher: | MDPI | ||||||
ISSN: | 1661-6596 | ||||||
Language: | English | ||||||
Publication Type: | Research article | ||||||
Article category: | Scientific peer reviewed | ||||||
Version: | Published version | ||||||
Copyright: | Creative Commons: Attribution 4.0 | ||||||
Full Text Status: | Public | ||||||
Subjects: | (A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology (A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Botany | ||||||
Keywords: | ABPP, apoplast, proteomics, serine hydrolases, beta-glycosidases, potato, field-omics | ||||||
URN:NBN: | urn:nbn:se:slu:epsilon-p-114711 | ||||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-114711 | ||||||
Additional ID: |
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ID Code: | 26312 | ||||||
Faculty: | LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap | ||||||
Department: | (LTJ, LTV) > Department of Plant Protection Biology | ||||||
Deposited By: | SLUpub Connector | ||||||
Deposited On: | 15 Dec 2021 15:26 | ||||||
Metadata Last Modified: | 15 Dec 2021 15:31 |
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