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Development of a 3-transcript host expression assay to differentiate between viral and bacterial infections in pigs

Hjertner, Bernt and Lützelschwab, Claudia and Schieck, Elise and Nzau, Benjamin and Henson, Sonal and Sjolund, Marie and Fossum, Caroline and Magnusson, Ulf (2021). Development of a 3-transcript host expression assay to differentiate between viral and bacterial infections in pigs. PLoS ONE. 16 , e0256106
[Research article]

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Abstract

Indiscriminate use of antibiotics to treat infections that are of viral origin contributes to unnecessary use which potentially may induce resistance in commensal bacteria. To counteract this a number of host gene transcriptional studies have been conducted to identify genes that are differently expressed during bacterial and viral infections in humans, and thus could be used as a tool to base decisions on the use of antibiotics. In this paper, we aimed to evaluate the potential of a selection of genes that have been considered biomarkers in humans, to differentially diagnose bacterial from viral infections in the pig. First porcine PBMC were induced with six toll-like receptor (TLR) agonists (FliC, LPS, ODN 2216, Pam3CSK4, poly I:C, R848) to mimic host gene expression induced by bacterial or viral pathogens, or exposed to heat-killed Actinobacillus pleuropneumoniae or a split influenza virus. Genes that were differentially expressed between bacterial and viral inducers were further evaluated on clinical material comprising eleven healthy pigs, and six pigs infected with A. pleuropneumoniae. This comprised three virally upregulated genes (IFI44L, MxA, RSAD2) and four bacterially upregulated genes (IL-1 beta, IL-8, FAM89A, S100PBP). All six infected pigs could be differentially diagnosed to healthy pigs using a host gene transcription assay based on the geometric average of the bacterially induced genes IL-8 and S100PBP over that of the virally induced gene MxA.

Authors/Creators:Hjertner, Bernt and Lützelschwab, Claudia and Schieck, Elise and Nzau, Benjamin and Henson, Sonal and Sjolund, Marie and Fossum, Caroline and Magnusson, Ulf
Title:Development of a 3-transcript host expression assay to differentiate between viral and bacterial infections in pigs
Series Name/Journal:PLoS ONE
Year of publishing :2021
Volume:16
Article number:e0256106
Number of Pages:14
Publisher:PUBLIC LIBRARY SCIENCE
ISSN:1932-6203
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
URN:NBN:urn:nbn:se:slu:epsilon-p-114885
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-114885
Additional ID:
Type of IDID
DOI10.1371/journal.pone.0256106
Web of Science (WoS)000725694500011
ID Code:26325
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Department of Biomedical Science and Veterinary Public Health
(VH) > Dept. of Clinical Sciences
Deposited By: SLUpub Connector
Deposited On:16 Dec 2021 15:25
Metadata Last Modified:16 Dec 2021 15:31

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