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Phytophthora infestans Ago1-associated miRNA promotes potato late blight disease

Hu, Xinyi and Persson Hodén, Kristian and Liao, Zhen and Åsman, Anna and Dixelius, Christina (2021). Phytophthora infestans Ago1-associated miRNA promotes potato late blight disease. New Phytologist. 233 , 443-457
[Research article]

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Abstract

Phytophthora spp. cause serious damage to plants by exploiting a large number of effector proteins and small RNAs (sRNAs). Several reports have described modulation of host RNA biogenesis and defence gene expression. Here, we analysed Phytophthora infestans Argonaute (Ago) 1 associated small RNAs during potato leaf infection. Small RNAs were co-immunoprecipitated, deep sequenced and analysed against the P. infestans and potato genomes, followed by transcript analyses and transgenic assays on a predicted target. Extensive targeting of potato and pathogen-derived sRNAs to a range of mRNAs was observed, including 638 sequences coding for resistance (R) proteins in the host genome. The single miRNA encoded by P. infestans (miR8788) was found to target a potato alpha/beta hydrolase-type encoding gene (StABH1), a protein localized to the plasma membrane. Analyses of stable transgenic potato lines harbouring overexpressed StABH1 or artificial miRNA gene constructs demonstrated the importance of StABH1 during infection by P. infestans. miR8788 knock-down strains showed reduced growth on potato, and elevated StABH1 expression levels were observed when plants were inoculated with the two knock-down strains compared to the wild-type strain 88069. The findings of our study suggest that sRNA encoded by P. infestans can affect potato mRNA, thereby expanding our knowledge of the multifaceted strategies this species uses to facilitate infection.

Authors/Creators:Hu, Xinyi and Persson Hodén, Kristian and Liao, Zhen and Åsman, Anna and Dixelius, Christina
Title:Phytophthora infestans Ago1-associated miRNA promotes potato late blight disease
Series Name/Journal:New Phytologist
Year of publishing :2021
Volume:233
Page range:443-457
Number of Pages:15
Publisher:WILEY
ISSN:0028-646X
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science
Keywords:Argonaute (Ago), microRNA (miRNA), Phytophthora infestans, small RNAs, Solanum tuberosum
URN:NBN:urn:nbn:se:slu:epsilon-p-114312
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-114312
Additional ID:
Type of IDID
DOI10.1111/nph.17758
Web of Science (WoS)000711583700001
ID Code:26343
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231)
Deposited By: SLUpub Connector
Deposited On:20 Dec 2021 06:25
Metadata Last Modified:20 Dec 2021 06:31

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