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Genomic and Phenotypic Characteristics in Geographically Separated Clinical Campylobacter jejuni ST353CC Isolates

Dicksved, Johan and Johansson, Cecilia and Kampmann, Christian and Nilsson, Anna and Dicksved, Johan and Engstrand, Lars and Rautelin, Hilpi (2021). Genomic and Phenotypic Characteristics in Geographically Separated Clinical Campylobacter jejuni ST353CC Isolates. Microorganisms. 9 , 2540
[Research article]

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Abstract

Campylobacter jejuni fecal isolates of eight international travelers, 5 of which had traveled to Ecuador and 3 to Bangladesh, were characterized, and the possible relationship between bacterial traits and clinical symptoms was further analyzed. All eight isolates belonged to the same MultiLocus Sequence Type clonal complex (ST353CC). The three isolates from Bangladesh were all of the same sequence type (ST-9438), and when compared to isolates of various other sequence types, they had a larger quantity of unique genetic content, higher expression levels of some putative virulence genes involved in adhesion and invasion (flpA, ciaB and iamA), and showed higher adhesion levels to human HT-29 colon cancer cells in an in vitro infection model. However, in contrast to the seemingly higher pathogenic potential of these bacterial isolates, travelers infected with the ST-9438 isolates had no or only very mild symptoms, whereas the other individuals, whose bacterial isolates seemed to have less pathogenic potential, generally reported severe symptoms. When studying the 16S rRNA gene-based fecal microbiota in samples collected prior to travel, there was an individual variation in the relative abundance of the three major bacterial phyla Actinobacteria, Bacteroidetes and Firmicutes, but there were no associations between composition and diversity of microbiota and development of severe symptoms from the infection. It remains to be confirmed by larger studies whether an individual’s characteristics such as gut microbiota, might be related to the severity of symptoms in Campylobacter infections.

Authors/Creators:Dicksved, Johan and Johansson, Cecilia and Kampmann, Christian and Nilsson, Anna and Dicksved, Johan and Engstrand, Lars and Rautelin, Hilpi
Title:Genomic and Phenotypic Characteristics in Geographically Separated Clinical Campylobacter jejuni ST353CC Isolates
Series Name/Journal:Microorganisms
Year of publishing :2021
Volume:9
Article number:2540
Number of Pages:11
ISSN:2076-2607
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Microbiology (Microbiology in the medical area to be 30109)
Keywords:campylobacter jejuni, ST353CC, virulence, pathogenesis, microbiota, whole-genome sequence
URN:NBN:urn:nbn:se:slu:epsilon-p-115003
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-115003
Additional ID:
Type of IDID
DOI10.3390/microorganisms9122540
ID Code:26398
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Animal Nutrition and Management
Deposited By: SLUpub Connector
Deposited On:23 Dec 2021 05:25
Metadata Last Modified:23 Dec 2021 06:01

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