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DNA fragmentation and membrane integrity in sperm of farmed Arctic charr (Salvelinus alpinus)

Jeuthe, Henrik and Palaiokostas, Christos and Johannisson, Anders (2022). DNA fragmentation and membrane integrity in sperm of farmed Arctic charr (Salvelinus alpinus). Aquaculture. 547 , 737537
[Research article]

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Abstract

Despite the relatively long history of captive breeding, the Arctic charr still exhibits a generally low, but highly variable reproductive performance in aquaculture. A recent publication exposed potential paternal factors influencing the reproductive outcome of Arctic charr broodstock from the Swedish breeding program. Interestingly, the paternal factor appeared to be more closely connected to embryo survival than to fertilisation rates. This lead to speculations on whether e.g. chromatin related issues, potentially related to oxidative stress could be involved. In order to investigate this hypothesis the present study assessed the levels of DNA fragmentation, using the SCD-method, and membrane integrity, using flow cytometry, in sperm of farmed Arctic charr. Moreover, the existence of associations was tested between DNA fragmentation and membrane integrity in individual semen samples and viability of their resulting progeny. We found high levels of DNA fragmentation in sperm from the Arctic charr sires, ranging from 24% to 86% with a median of 67%. Membrane integrity values were high, with individual levels of 93.1% to 99.6% viable sperm cells, median 98.8%. DNA fragmentation and membrane integrity values were moderately correlated (r = 0.304, p < 0.05). Fertilisation rates and proportions of eyed eggs showed substantial individual variation and were correlated (r = 0.497, p < 0.05). However, large differences between proportion of eyed eggs and fertilisation rate, median 52% and 81.6% respectively, highlight that the main loss occurred due to embryo mortality rather than failed fertilisation. No correlation was found between either DNA fragmentation or membrane integrity and the resulting reproductive outcome (fertilisation rate and eyed eggs) of the individual Arctic charr sires. Overall, our study identified very high levels of DNA fragmentation, which could influence the fertility of the broodstock in question and thereby be a mitigating mechanism involved in the low reproductive success most often observed in farmed Arctic charr. Further exploration of this relationship would be needed, though.

Authors/Creators:Jeuthe, Henrik and Palaiokostas, Christos and Johannisson, Anders
Title:DNA fragmentation and membrane integrity in sperm of farmed Arctic charr (Salvelinus alpinus)
Series Name/Journal:Aquaculture
Year of publishing :2022
Volume:547
Article number:737537
Number of Pages:9
Publisher:ELSEVIER
ISSN:0044-8486
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Fish and Aquacultural Science
Keywords:Chromatin, Seasonal change, Sperm quality, Oxidative stress, Salmonid, Reproduction
URN:NBN:urn:nbn:se:slu:epsilon-p-115051
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-115051
Additional ID:
Type of IDID
DOI10.1016/j.aquaculture.2021.737537
Web of Science (WoS)000729123800005
ID Code:26600
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Animal Breeding and Genetics
(VH) > Dept. of Clinical Sciences
Deposited By: SLUpub Connector
Deposited On:27 Dec 2021 05:26
Metadata Last Modified:27 Dec 2021 06:01

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