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Reprogramming of RNA silencing triggered by cucumber mosaic virus infection in Arabidopsis

Annacondia, María Luz and Martinez Arias, German (2021). Reprogramming of RNA silencing triggered by cucumber mosaic virus infection in Arabidopsis. Genome Biology. 22 , 340
[Research article]

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Abstract

Background RNA silencing has an important role mediating sequence-specific virus resistance in plants. The complex interaction of viruses with RNA silencing involves the loading of viral small interfering RNAs (vsiRNAs) into its host ARGONAUTE (AGO) proteins. As a side effect of their antiviral activity, vsiRNAs loading into AGO proteins can also mediate the silencing of endogenous genes. Here, we analyze at the genome-wide level both aspects of the interference of cucumber mosaic virus (CMV) with the RNA silencing machinery of Arabidopsis thaliana. Results We observe CMV-derived vsiRNAs affect the levels of endogenous sRNA classes. Furthermore, we analyze the incorporation of vsiRNAs into AGO proteins with a described antiviral role and the viral suppressor of RNA silencing (VSR) 2b, by combining protein immunoprecipitation with sRNA high-throughput sequencing. Interestingly, vsiRNAs represent a substantial percentage of AGO-loaded sRNAs and displace other endogenous sRNAs. As a countermeasure, the VSR 2b loaded vsiRNAs and mRNA-derived siRNAs, which affect the expression of the genes they derive from. Additionally, we analyze how vsiRNAs incorporate into the endogenous RNA silencing pathways by exploring their target mRNAs using parallel analysis of RNA end (PARE) sequencing, which allow us to identify vsiRNA-targeted genes genome-wide. Conclusions This work exemplifies the complex relationship of RNA viruses with the endogenous RNA silencing machinery and the multiple aspects of virus resistance and virulence that this interaction induces.

Authors/Creators:Annacondia, María Luz and Martinez Arias, German
Title:Reprogramming of RNA silencing triggered by cucumber mosaic virus infection in Arabidopsis
Series Name/Journal:Genome Biology
Year of publishing :2021
Volume:22
Article number:340
Number of Pages:22
Publisher:BMC
ISSN:1474-760X
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Plant Biotechnology
Keywords:RNA silencing, Virus, Argonaute, Immunoprecipitation, Cucumber mosaic virus
URN:NBN:urn:nbn:se:slu:epsilon-p-115057
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-115057
Additional ID:
Type of IDID
DOI10.1186/s13059-021-02564-z
Web of Science (WoS)000730581500001
ID Code:26606
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231)
Deposited By: SLUpub Connector
Deposited On:27 Dec 2021 07:25
Metadata Last Modified:27 Dec 2021 07:31

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