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Quantification of normetanephrine in canine urine using ELISA: evaluation of factors affecting results

Höglund, Katja and Ringmark, Sara and Palmqvist, Hanna and Svensson, Anna (2022). Quantification of normetanephrine in canine urine using ELISA: evaluation of factors affecting results. Journal of Veterinary Diagnostic Investigation. 34 , 10406387211052984 , 28-35
[Research article]

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Abstract

Catecholamine release increases in dogs with pheochromocytomas and in situations of stress. Although plasma catecholamines degrade rapidly, their metabolites, normetanephrine (NME) and metanephrine (ME), are stable in acidified urine. Our aim was to verify a human urine ELISA kit for the quantification of NME and ME in canine urine and to determine the effects on metabolite stability of sampling time (morning or midday) and day (ordinary or day spent in a clinic). We analyzed 179 urine samples from 17 healthy dogs. For NME, the mean intra-assay CV was 6.0% for all samples and 4.3% for the canine control; inter-assay CVs were 3.3, 3.8, and 12% for high and low concentration human urine positive controls supplied in the ELISA kit and a positive canine control, respectively; spike-recovery was 90-101%. For ME, mean intra-assay CV was 6.5% for samples and 9.0% for the canine control; inter-assay CVs were 12.7, 7.2, and 22.5% for high and low concentration human urine positive controls supplied in the ELISA kit and a positive canine control, respectively; spike-recovery was 85-89%. Dilution recovery was unsatisfactory for both metabolites. Based on our verification results, NME was selected for remaining analyses. We found no effect on NME concentrations of acidification or room temperature storage for up to 24 h. The NME:creatinine ratio was higher after the first of 3 clinic days compared to the same morning (111.2 +/- 5.5 vs. 82.9 +/- 5.3; p < 0.0001), but not on the other days. NME verification results were generally superior to ME. Dilution studies were unsatisfactory for both metabolites. Given that NME was stable without acidification at room temperature, urine samples can be collected at home. The clinic environment can cause higher NME:creatinine ratios, especially in unaccustomed dogs.

Authors/Creators:Höglund, Katja and Ringmark, Sara and Palmqvist, Hanna and Svensson, Anna
Title:Quantification of normetanephrine in canine urine using ELISA: evaluation of factors affecting results
Series Name/Journal:Journal of Veterinary Diagnostic Investigation
Year of publishing :2022
Volume:34
Article number:10406387211052984
Number of Pages:8
Publisher:SAGE PUBLICATIONS INC
ISSN:1040-6387
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Pathobiology
Keywords:acidification, catecholamine, dogs, metanephrine, metanephrines, normetanephrine, sample storage
URN:NBN:urn:nbn:se:slu:epsilon-p-114300
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-114300
Additional ID:
Type of IDID
DOI10.1177/10406387211052984
Web of Science (WoS)000711419200001
ID Code:26627
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Anatomy, Physiology and Biochemistry
(VH) > Dept. of Animal Nutrition and Management
(VH) > Dept. of Clinical Sciences
Deposited By: SLUpub Connector
Deposited On:28 Dec 2021 04:25
Metadata Last Modified:28 Dec 2021 06:01

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