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Polymorphisms in the serotonin transporter gene and circulating concentrations of neurotransmitters in Cavalier King Charles Spaniels with myxomatous mitral valve disease

Reimann, Maria J. and Fredholm, Merete and Cremer, Signe E. and Christiansen, Liselotte B. and Meurs, Kathryn M. and Moller, Jacob E. and Häggström, Jens and Lykkesfeldt, Jens and Olsen, Lisbeth H. (2021). Polymorphisms in the serotonin transporter gene and circulating concentrations of neurotransmitters in Cavalier King Charles Spaniels with myxomatous mitral valve disease. Journal of Veterinary Internal Medicine. 35 , 2596-2606
[Research article]

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Abstract

Background The neurotransmitter serotonin (5-HT) affects valvular degeneration and dogs with myxomatous mitral valve disease (MMVD) exhibit alterations in 5-HT signaling. In Maltese dogs, 3 single nucleotide polymorphisms (SNPs) in the 5-HT transporter (SERT) gene are suggested to associate with MMVD. Hypothesis/Objectives Determine the association of SERT polymorphisms on MMVD severity and serum 5-HT concentration in Cavalier King Charles Spaniels (CKCS). Additionally, investigate the association between selected clinical and hematologic variables and serum 5-HT and assess the correlation between HPLC and ELISA measurements of serum 5-HT. Animals Seventy-one CKCS (42 females and 29 males; 7.8 [4.7;9.9] years (median [Q1;Q3])) in different MMVD stages. Methods This prospective study used TaqMan genotyping assays to assess SERT gene polymorphisms. Neurotransmitter concentrations were assessed by HPLC and ELISA. Results TaqMan analyses identified none of the selected SERT polymorphisms in any of the CKCS examined. Serum 5-HT was associated with platelet count (P < .001) but not MMVD severity, age or medical therapy and did not correlate with serum concentration of the 5-HT metabolite, 5-hydroxyindoleacetic acid. The ELISA serum 5-HT correlated with HPLC measurements (rho = .87; P < .0001) but was lower (mean difference = -22 ng/mL; P = .02) independent of serum 5-HT concentration (P = .2). Conclusions and Clinical Importance Selected SERT SNPs associated with MMVD in Maltese dogs were not found in CKCS and only platelet count influenced serum 5-HT concentration. These SNPs are unlikely to be associated with MMVD pathophysiology or serum 5-HT concentration in CKCS. HPLC and ELISA serum 5-HT demonstrated good correlation but ELISA systematically underestimated 5-HT.

Authors/Creators:Reimann, Maria J. and Fredholm, Merete and Cremer, Signe E. and Christiansen, Liselotte B. and Meurs, Kathryn M. and Moller, Jacob E. and Häggström, Jens and Lykkesfeldt, Jens and Olsen, Lisbeth H.
Title:Polymorphisms in the serotonin transporter gene and circulating concentrations of neurotransmitters in Cavalier King Charles Spaniels with myxomatous mitral valve disease
Series Name/Journal:Journal of Veterinary Internal Medicine
Year of publishing :2021
Volume:35
Page range:2596-2606
Number of Pages:11
Publisher:WILEY
ISSN:0891-6640
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution-Noncommercial-No Derivative Works 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
Keywords:dogs, ELISA, genetic variation, heart disease, HPLC, serum serotonin
URN:NBN:urn:nbn:se:slu:epsilon-p-113971
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-113971
Additional ID:
Type of IDID
DOI10.1111/jvim.16277
Web of Science (WoS)000702731900001
ID Code:26630
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
Deposited By: SLUpub Connector
Deposited On:28 Dec 2021 04:25
Metadata Last Modified:28 Dec 2021 06:01

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