Balinandi, Stephen and von Bromssen, Claudia and Tumusiime, Alex and Kyondo, Jackson and Kwon, Hyesoo and Monteil, Vanessa M. and Mirazimi, Ali and Lutwama, Julius and Mugisha, Lawrence and Malmberg, Maja
(2021).
Serological and molecular study of Crimean-Congo Hemorrhagic Fever Virus in cattle from selected districts in Uganda.
Journal of Virological Methods. 290
, 114075
[Research article]
![]() |
PDF
3MB |
Abstract
Background: Crimean-Congo Hemorrhagic Fever (CCHF) is a severe tick-borne viral hemorrhagic disease caused by Crimean-Congo Hemorrhagic Fever Virus (CCHFV) that poses serious public health challenges in many parts of Africa, Europe and Asia.Methods: We examined 500 cattle sera samples from five districts for CCHFV antibodies using in-house and commercially available (IDVet) ELISA, Immunofluorescent assay (IFA) and Real-time polymerase chain reaction (RT-PCR).Results: 500 cattle (73.8 % females) were analyzed; CCHFV seropositivity was 12.6 % (n = 63) and 75.0 % (n = 375) with the in-house and IDVet ELISAs, respectively. Seropositivity was associated with geographical location, increasing age, being female, and having a higher tick burden. Twenty four out of the 37 (64.8 %) were seropositive for CCHFV using IFA and all were negative for virus on RT-PCR. The IFA results were more com-parable to IDVet (kappa(coefficient) = 0.88, p = <0.01) than to in-house (kappa(coefficient) = 0.32, p = 0.02).Conclusions: Our study confirmed the presence and high prevalence of anti-CCHF antibodies in cattle based on three methods from all the five study districts, confirming presence and exposure of CCHFV. Given the zoonotic potential for CCHFV, we recommend a multidisciplinary public health surveillance and epidemiology of CCHFV in both animals and humans throughout the country.
Authors/Creators: | Balinandi, Stephen and von Bromssen, Claudia and Tumusiime, Alex and Kyondo, Jackson and Kwon, Hyesoo and Monteil, Vanessa M. and Mirazimi, Ali and Lutwama, Julius and Mugisha, Lawrence and Malmberg, Maja | ||||||
---|---|---|---|---|---|---|---|
Title: | Serological and molecular study of Crimean-Congo Hemorrhagic Fever Virus in cattle from selected districts in Uganda | ||||||
Series Name/Journal: | Journal of Virological Methods | ||||||
Year of publishing : | 2021 | ||||||
Volume: | 290 | ||||||
Article number: | 114075 | ||||||
Number of Pages: | 9 | ||||||
Publisher: | ELSEVIER | ||||||
ISSN: | 0166-0934 | ||||||
Language: | English | ||||||
Publication Type: | Research article | ||||||
Article category: | Scientific peer reviewed | ||||||
Version: | Published version | ||||||
Copyright: | Creative Commons: Attribution 4.0 | ||||||
Full Text Status: | Public | ||||||
Subjects: | (A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science | ||||||
Keywords: | Crimean-Congo Hemorrhagic Fever Virus, Seroprevalence, Cattle, Uganda, Immunofluorescent assay | ||||||
URN:NBN: | urn:nbn:se:slu:epsilon-p-114786 | ||||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-114786 | ||||||
Additional ID: |
| ||||||
ID Code: | 26633 | ||||||
Faculty: | VH - Faculty of Veterinary Medicine and Animal Science | ||||||
Department: | (VH) > Department of Biomedical Science and Veterinary Public Health (VH) > Dept. of Animal Breeding and Genetics | ||||||
Deposited By: | SLUpub Connector | ||||||
Deposited On: | 28 Dec 2021 06:25 | ||||||
Metadata Last Modified: | 28 Dec 2021 06:31 |
Repository Staff Only: item control page