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Serological and molecular study of Crimean-Congo Hemorrhagic Fever Virus in cattle from selected districts in Uganda

Balinandi, Stephen and von Bromssen, Claudia and Tumusiime, Alex and Kyondo, Jackson and Kwon, Hyesoo and Monteil, Vanessa M. and Mirazimi, Ali and Lutwama, Julius and Mugisha, Lawrence and Malmberg, Maja (2021). Serological and molecular study of Crimean-Congo Hemorrhagic Fever Virus in cattle from selected districts in Uganda. Journal of Virological Methods. 290 , 114075
[Research article]

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Abstract

Background: Crimean-Congo Hemorrhagic Fever (CCHF) is a severe tick-borne viral hemorrhagic disease caused by Crimean-Congo Hemorrhagic Fever Virus (CCHFV) that poses serious public health challenges in many parts of Africa, Europe and Asia.Methods: We examined 500 cattle sera samples from five districts for CCHFV antibodies using in-house and commercially available (IDVet) ELISA, Immunofluorescent assay (IFA) and Real-time polymerase chain reaction (RT-PCR).Results: 500 cattle (73.8 % females) were analyzed; CCHFV seropositivity was 12.6 % (n = 63) and 75.0 % (n = 375) with the in-house and IDVet ELISAs, respectively. Seropositivity was associated with geographical location, increasing age, being female, and having a higher tick burden. Twenty four out of the 37 (64.8 %) were seropositive for CCHFV using IFA and all were negative for virus on RT-PCR. The IFA results were more com-parable to IDVet (kappa(coefficient) = 0.88, p = <0.01) than to in-house (kappa(coefficient) = 0.32, p = 0.02).Conclusions: Our study confirmed the presence and high prevalence of anti-CCHF antibodies in cattle based on three methods from all the five study districts, confirming presence and exposure of CCHFV. Given the zoonotic potential for CCHFV, we recommend a multidisciplinary public health surveillance and epidemiology of CCHFV in both animals and humans throughout the country.

Authors/Creators:Balinandi, Stephen and von Bromssen, Claudia and Tumusiime, Alex and Kyondo, Jackson and Kwon, Hyesoo and Monteil, Vanessa M. and Mirazimi, Ali and Lutwama, Julius and Mugisha, Lawrence and Malmberg, Maja
Title:Serological and molecular study of Crimean-Congo Hemorrhagic Fever Virus in cattle from selected districts in Uganda
Series Name/Journal:Journal of Virological Methods
Year of publishing :2021
Volume:290
Article number:114075
Number of Pages:9
Publisher:ELSEVIER
ISSN:0166-0934
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
Keywords:Crimean-Congo Hemorrhagic Fever Virus, Seroprevalence, Cattle, Uganda, Immunofluorescent assay
URN:NBN:urn:nbn:se:slu:epsilon-p-114786
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-114786
Additional ID:
Type of IDID
DOI10.1016/j.jviromet.2021.114075
Web of Science (WoS)000632515400007
ID Code:26633
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Department of Biomedical Science and Veterinary Public Health
(VH) > Dept. of Animal Breeding and Genetics
Deposited By: SLUpub Connector
Deposited On:28 Dec 2021 06:25
Metadata Last Modified:28 Dec 2021 06:31

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