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High-yield production of a super-soluble miniature spidroin for biomimetic high-performance materials

Schmuck, Benjamin and Greco, Gabriele and Barth, Andreas and Pugno, Nicola M. and Johansson, Jan and Rising, Anna (2021). High-yield production of a super-soluble miniature spidroin for biomimetic high-performance materials. Materials Today. 50 , 16-23
[Research article]

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Abstract

The mechanical properties of artificial spider silks are approaching a stage where commercial applications become realistic. However, the yields of recombinant silk proteins that can be used to produce fibers with good mechanical properties are typically very low and many purification and spinning protocols still require the use of urea, hexafluoroisopropanol, and/or methanol. Thus, improved production and spinning methods with a minimal environmental impact are needed. We have previously developed a miniature spider silk protein that is characterized by high solubility in aqueous buffers and spinnability in biomimetic set-ups. In this study, we developed a production protocol that resulted in an expression level of >20 g target protein per liter in an Escherichia coli fedbatch culture, and subsequent purification under native conditions yielded 14.5 g/l. This corresponds to a nearly six-fold increase in expression levels, and a 10-fold increase in yield after purification compared to reports for recombinant spider silk proteins. Biomimetic spinning using only aqueous buffers resulted in fibers with a toughness modulus of 74 MJ/m(3), which is the highest reported for biomimetically as-spun artificial silk fibers. Thus, the process described herein represents a milestone for the economic production of biomimetic silk fibers for industrial applications.

Authors/Creators:Schmuck, Benjamin and Greco, Gabriele and Barth, Andreas and Pugno, Nicola M. and Johansson, Jan and Rising, Anna
Title:High-yield production of a super-soluble miniature spidroin for biomimetic high-performance materials
Series Name/Journal:Materials Today
Year of publishing :2021
Volume:50
Page range:16-23
Number of Pages:8
Publisher:ELSEVIER SCI LTD
ISSN:1369-7021
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 2 Engineering and Technology > 209 Industrial Biotechnology > Bio Materials
Keywords:Artificial spider silk, Spidroins, Bioreactor cultivation, Recombinant protein expression
URN:NBN:urn:nbn:se:slu:epsilon-p-115312
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-115312
Additional ID:
Type of IDID
DOI10.1016/j.mattod.2021.07.020
Web of Science (WoS)000731402500007
ID Code:26696
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Anatomy, Physiology and Biochemistry
Deposited By: SLUpub Connector
Deposited On:14 Jan 2022 09:25
Metadata Last Modified:12 Sep 2022 21:18

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