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A Verticillium longisporum pleiotropic drug transporter determines tolerance to the plant host beta-pinene monoterpene

Rafiei, Vahideh and Ruffino, Alessandra and Persson Hodén, Kristian and Törnkvist, Anna and Mozuraitis, Raimondas and Dubey, Mukesh and Tzelepis, Georgios (2021). A Verticillium longisporum pleiotropic drug transporter determines tolerance to the plant host beta-pinene monoterpene. Molecular Plant Pathology. 23 , 291-303
[Research article]

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Abstract

Terpenes constitute a major part of secondary metabolites secreted by plants in the rhizosphere. However, their specific functions in fungal-plant interactions have not been investigated thoroughly. In this study we investigated the role of monoterpenes in interactions between oilseed rape (Brassica napus) and the soilborne pathogen Verticillium longisporum. We identified seven monoterpenes produced by B. napus, and production of alpha-pinene, beta-pinene, 3-carene, and camphene was significantly increased upon fungal infection. Among them, beta-pinene was chosen for further analysis. Transcriptome analysis of V. longisporum on exposure to beta-pinene resulted in identification of two highly expressed pleotropic drug transporters paralog genes named VlAbcG1a and VlAbcG1b. Overexpression of VlAbcG1a in Saccharomyces cerevisiae increased tolerance to beta-pinene, while deletion of the VlAbcG1a homologous gene in Verticillium dahliae resulted in mutants with increased sensitivity to certain monoterpenes. Furthermore, the VlAbcG1a overexpression strain displayed an increased tolerance to beta-pinene and increased virulence in tomato plants. Data from this study give new insights into the roles of terpenes in plant-fungal pathogen interactions and the mechanisms fungi deploy to cope with the toxicity of these secondary metabolites.

Authors/Creators:Rafiei, Vahideh and Ruffino, Alessandra and Persson Hodén, Kristian and Törnkvist, Anna and Mozuraitis, Raimondas and Dubey, Mukesh and Tzelepis, Georgios
Title:A Verticillium longisporum pleiotropic drug transporter determines tolerance to the plant host beta-pinene monoterpene
Series Name/Journal:Molecular Plant Pathology
Year of publishing :2021
Volume:23
Page range:291-303
Number of Pages:13
Publisher:WILEY
ISSN:1464-6722
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution-Noncommercial-No Derivative Works 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Botany
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology
Keywords:ABC-transporters, Brassica napus, monoterpenes, soilborne pathogen, beta-pinene
URN:NBN:urn:nbn:se:slu:epsilon-p-114705
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-114705
Additional ID:
Type of IDID
DOI10.1111/mpp.13162
Web of Science (WoS)000722609900001
ID Code:26745
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
S - Faculty of Forest Sciences
Department:(NL, NJ) > Dept. of Forest Mycology and Plant Pathology
(S) > Dept. of Forest Mycology and Plant Pathology

(NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231)
Deposited By: SLUpub Connector
Deposited On:17 Jan 2022 14:25
Metadata Last Modified:13 Sep 2022 19:57

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