Hasby, Fahri and Barbi, Florian and Manzoni, Stefano and Lindahl, Björn
(2021).
Transcriptomic markers of fungal growth, respiration and carbon-use efficiency.
FEMS Microbiology Letters. 368
, fnab100
[Research article]
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Abstract
Fungal metabolic carbon acquisition and its subsequent partitioning between biomass production and respiration, i.e. the carbon-use efficiency (CUE), are central parameters in biogeochemical modeling. However, current available techniques for estimating these parameters are all associated with practical and theoretical shortcomings, making assessments unreliable. Gene expression analyses hold the prospect of phenotype prediction by indirect means, providing new opportunities to obtain information about metabolic priorities. We cultured four different fungal isolates (Chalara longipes, Laccaria bicolor, Serpula lacrymans and Trichoderma harzianum) in liquid media with contrasting nitrogen availability and measured growth rates and respiration to calculate CUE. By relating gene expression markers to measured carbon fluxes, we identified genes coding for 1,3-β-glucan synthase and 2-oxoglutarate dehydrogenase as suitable markers for growth and respiration, respectively, capturing both intraspecific variation as well as within-strain variation dependent on growth medium. A transcript index based on these markers correlated significantly with differences in CUE between the fungal isolates. Our study paves the way for the use of these markers to assess differences in growth, respiration and CUE in natural fungal communities, using metatranscriptomic or the RT-qPCR approach.
Authors/Creators: | Hasby, Fahri and Barbi, Florian and Manzoni, Stefano and Lindahl, Björn | ||||||
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Title: | Transcriptomic markers of fungal growth, respiration and carbon-use efficiency | ||||||
Series Name/Journal: | FEMS Microbiology Letters | ||||||
Year of publishing : | 2021 | ||||||
Volume: | 368 | ||||||
Article number: | fnab100 | ||||||
Number of Pages: | 9 | ||||||
ISSN: | 0378-1097 | ||||||
Language: | English | ||||||
Publication Type: | Research article | ||||||
Article category: | Scientific peer reviewed | ||||||
Version: | Published version | ||||||
Copyright: | Creative Commons: Attribution 4.0 | ||||||
Full Text Status: | Public | ||||||
Subjects: | (A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology (A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Microbiology (Microbiology in the medical area to be 30109) (A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Ecology | ||||||
URN:NBN: | urn:nbn:se:slu:epsilon-p-115525 | ||||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-115525 | ||||||
Additional ID: |
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Alternative URL: | https://academic.oup.com/femsle/article/368/15/fnab100/6335482 | ||||||
ID Code: | 26755 | ||||||
Faculty: | NJ - Fakulteten för naturresurser och jordbruksvetenskap | ||||||
Department: | (NL, NJ) > Dept. of Soil and Environment (S) > Dept. of Soil and Environment | ||||||
Deposited By: | SLUpub Connector | ||||||
Deposited On: | 20 Jan 2022 06:25 | ||||||
Metadata Last Modified: | 16 May 2022 20:38 |
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