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Transcriptomic markers of fungal growth, respiration and carbon-use efficiency

Hasby, Fahri and Barbi, Florian and Manzoni, Stefano and Lindahl, Björn (2021). Transcriptomic markers of fungal growth, respiration and carbon-use efficiency. FEMS Microbiology Letters. 368 , fnab100
[Research article]

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Abstract

Fungal metabolic carbon acquisition and its subsequent partitioning between biomass production and respiration, i.e. the carbon-use efficiency (CUE), are central parameters in biogeochemical modeling. However, current available techniques for estimating these parameters are all associated with practical and theoretical shortcomings, making assessments unreliable. Gene expression analyses hold the prospect of phenotype prediction by indirect means, providing new opportunities to obtain information about metabolic priorities. We cultured four different fungal isolates (Chalara longipes, Laccaria bicolor, Serpula lacrymans and Trichoderma harzianum) in liquid media with contrasting nitrogen availability and measured growth rates and respiration to calculate CUE. By relating gene expression markers to measured carbon fluxes, we identified genes coding for 1,3-β-glucan synthase and 2-oxoglutarate dehydrogenase as suitable markers for growth and respiration, respectively, capturing both intraspecific variation as well as within-strain variation dependent on growth medium. A transcript index based on these markers correlated significantly with differences in CUE between the fungal isolates. Our study paves the way for the use of these markers to assess differences in growth, respiration and CUE in natural fungal communities, using metatranscriptomic or the RT-qPCR approach.

Authors/Creators:Hasby, Fahri and Barbi, Florian and Manzoni, Stefano and Lindahl, Björn
Title:Transcriptomic markers of fungal growth, respiration and carbon-use efficiency
Series Name/Journal:FEMS Microbiology Letters
Year of publishing :2021
Volume:368
Article number:fnab100
Number of Pages:9
ISSN:0378-1097
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Microbiology (Microbiology in the medical area to be 30109)
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Ecology
URN:NBN:urn:nbn:se:slu:epsilon-p-115525
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-115525
Additional ID:
Type of IDID
DOI10.1093/femsle/fnab100
Otherhttps://academic.oup.com/femsle/article/368/15/fnab100/6335482
Alternative URL:https://academic.oup.com/femsle/article/368/15/fnab100/6335482
ID Code:26755
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Soil and Environment
(S) > Dept. of Soil and Environment
Deposited By: SLUpub Connector
Deposited On:20 Jan 2022 06:25
Metadata Last Modified:16 May 2022 20:38

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