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Rapid emergence of boscalid resistance in Swedish populations of Alternaria solani revealed by a combination of field and laboratory experiments

Mostafanezhad, Hadis and Edin, Eva and Grenville-Briggs Didymus, Laura and Lankinen, Åsa and Liljeroth, Erland (2022). Rapid emergence of boscalid resistance in Swedish populations of Alternaria solani revealed by a combination of field and laboratory experiments. European Journal of Plant Pathology. 162 :2 , 289-303
[Research article]

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Abstract

Early blight, caused by Alternaria solani, is a common potato disease worldwide. Reduced field efficacy of the fungicide boscalid against this disease has been reported in several countries. Boscalid resistance has been mostly studied with in-vitro and/or greenhouse experiments. Field studies validating this phenomenon are largely missing. Here, for the first time in Scandinavia, we validated boscalid resistance in a Swedish population of A. solani both in the field and in the laboratory. Field trials between 2014 and 2017 in Nymo showed significant efficacy reduction by year. The target regions of the A. solani genes encoding the succinate dehydrogenase subunits (Sdh) B, C and D of samples collected from Nymo, and additional fields in south-eastern and central Sweden, were analysed for substitutions associated with loss of boscalid sensitivity. In 2014, the SdhC-H134R mutation was found at several sites at a low frequency, while, in 2017, the majority of the samples had either the SdhB-H278Y or the SdhC-H134R substitution. No mutations were detected in the gene encoding the SdhD subunit. Spore germination tests showed a high sensitivity (EC50 < 1 mu g mL(-1)) of isolates lacking the substitutions. This was supported by a significant decrease in their radial growth rate, from 0.1 to 10 mu g mL(-1) boscalid. However, the mutated isolates had EC50 > 100 mu g mL(-1) and their growth rates hardly decreased at concentrations above 1-10 mu g mL(-1). These results add to the current knowledge of fungicide resistance development in field and indicate that early blight management in southeast Sweden should no longer rely on boscalid.

Authors/Creators:Mostafanezhad, Hadis and Edin, Eva and Grenville-Briggs Didymus, Laura and Lankinen, Åsa and Liljeroth, Erland
Title:Rapid emergence of boscalid resistance in Swedish populations of Alternaria solani revealed by a combination of field and laboratory experiments
Series Name/Journal:European Journal of Plant Pathology
Year of publishing :2022
Volume:162
Number:2
Page range:289-303
Number of Pages:15
Publisher:SPRINGER
ISSN:0929-1873
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science
Keywords:Early blight, Potato, Succinate dehydrogenase inhibitor (SDHI), Monitoring, Fungicide resistance, EC50
URN:NBN:urn:nbn:se:slu:epsilon-p-114255
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-114255
Additional ID:
Type of IDID
DOI10.1007/s10658-021-02403-8
Web of Science (WoS)000710060800001
ID Code:27086
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(LTJ, LTV) > Department of Plant Protection Biology
(NL, NJ) > Dept. of Forest Mycology and Plant Pathology
(S) > Dept. of Forest Mycology and Plant Pathology
Deposited By: SLUpub Connector
Deposited On:14 Feb 2022 12:25
Metadata Last Modified:14 Feb 2022 12:31

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