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Comprehensive analysis of chemical and biological problems associated with browning agents used in aquatic studies

Scharnweber, Kristin and Peura, Sari and Attermeyer, Katrin and Bertilsson, Stefan and Bolender, Lucas and Buck, Moritz and Einarsdottir, Karolina and Garcia, Sarahi L. and Gollnisch, Raphael and Grasset, Charlotte and Groeneveld, Marloes and Hawkes, Jeffrey A. and Lindstrom, Eva S. and Manthey, Christin and Overgaard, Robyn and Rengefors, Karin and Sedano-Nunez, Vicente T. and Tranvik, Lars J. and Székely, Anna (2021). Comprehensive analysis of chemical and biological problems associated with browning agents used in aquatic studies. Limnology and Oceanography: Methods. 19 :12 , 818-835
[Research article]

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Abstract

Inland waters receive and process large amounts of colored organic matter from the terrestrial surroundings. These inputs dramatically affect the chemical, physical, and biological properties of water bodies, as well as their roles as global carbon sinks and sources. However, manipulative studies, especially at ecosystem scale, require large amounts of dissolved organic matter with optical and chemical properties resembling indigenous organic matter. Here, we compared the impacts of two leonardite products (HuminFeed and SuperHume) and a freshly derived reverse osmosis concentrate of organic matter in a set of comprehensive mesocosm- and laboratory-scale experiments and analyses. The chemical properties of the reverse osmosis concentrate and the leonardite products were very different, with leonardite products being low and the reverse osmosis concentrate being high in carboxylic functional groups. Light had a strong impact on the properties of leonardite products, including loss of color and increased particle formation. HuminFeed presented a substantial impact on microbial communities under light conditions, where bacterial production was stimulated and community composition modified, while in dark potential inhibition of bacterial processes was detected. While none of the browning agents inhibited the growth of the tested phytoplankton Gonyostomum semen, HuminFeed had detrimental effects on zooplankton abundance and Daphnia reproduction. We conclude that the effects of browning agents extracted from leonardite, particularly HuminFeed, are in sharp contrast to those originating from terrestrially derived dissolved organic matter. Hence, they should be used with great caution in experimental studies on the consequences of terrestrial carbon for aquatic systems.

Authors/Creators:Scharnweber, Kristin and Peura, Sari and Attermeyer, Katrin and Bertilsson, Stefan and Bolender, Lucas and Buck, Moritz and Einarsdottir, Karolina and Garcia, Sarahi L. and Gollnisch, Raphael and Grasset, Charlotte and Groeneveld, Marloes and Hawkes, Jeffrey A. and Lindstrom, Eva S. and Manthey, Christin and Overgaard, Robyn and Rengefors, Karin and Sedano-Nunez, Vicente T. and Tranvik, Lars J. and Székely, Anna
Title:Comprehensive analysis of chemical and biological problems associated with browning agents used in aquatic studies
Series Name/Journal:Limnology and Oceanography: Methods
Year of publishing :2021
Volume:19
Number:12
Page range:818-835
Number of Pages:18
Publisher:WILEY
ISSN:1541-5856
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution-Noncommercial 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 105 Earth and Related Environmental Sciences > Oceanography, Hydrology, Water Resources
URN:NBN:urn:nbn:se:slu:epsilon-p-114309
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-114309
Additional ID:
Type of IDID
DOI10.1002/lom3.10463
Web of Science (WoS)000711887000001
ID Code:27127
Faculty:S - Faculty of Forest Sciences
NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Forest Mycology and Plant Pathology
(S) > Dept. of Forest Mycology and Plant Pathology

(NL, NJ) > Dept. of Aquatic Sciences and Assessment
Deposited By: SLUpub Connector
Deposited On:17 Feb 2022 13:24
Metadata Last Modified:17 Feb 2022 13:31

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