Kolbeck, Andreas and Marhavy, Peter and De Bellis, Damien and Li, Baohai and Kamiya, Takehiro and Fujiwara, Toru and Kalmbach, Lothar and Geldner, Niko
(2022).
CASP microdomain formation requires cross cell wall stabilization of domains and non-cell autonomous action of LOTR1.
eLife. 11
, e69602
[Research article]
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Abstract
Efficient uptake of nutrients in both animal and plant cells requires tissue-spanning diffusion barriers separating inner tissues from the outer lumen/soil. However, we poorly understand how such contiguous three-dimensional superstructures are formed in plants. Here, we show that correct establishment of the plant Casparian Strip (CS) network relies on local neighbor communication. We show that positioning of Casparian Strip membrane domains (CSDs) is tightly coordinated between neighbors in wild-type and that restriction of domain formation involves the putative extracellular protease LOTR1. Impaired domain restriction in lotr1 leads to fully functional CSDs at ectopic positions, forming 'half strips'. LOTR1 action in the endodermis requires its expression in the stele. LOTR1 endodermal expression cannot complement, while cortex expression causes a dominant-negative phenotype. Our findings establish LOTR1 as a crucial player in CSD positioning acting in a directional, non-cell-autonomous manner to restrict and coordinate CS positioning.
Authors/Creators: | Kolbeck, Andreas and Marhavy, Peter and De Bellis, Damien and Li, Baohai and Kamiya, Takehiro and Fujiwara, Toru and Kalmbach, Lothar and Geldner, Niko | ||||||
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Title: | CASP microdomain formation requires cross cell wall stabilization of domains and non-cell autonomous action of LOTR1 | ||||||
Series Name/Journal: | eLife | ||||||
Year of publishing : | 2022 | ||||||
Volume: | 11 | ||||||
Article number: | e69602 | ||||||
Number of Pages: | 21 | ||||||
Publisher: | eLIFE SCIENCES PUBL LTD | ||||||
ISSN: | 2050-084X | ||||||
Language: | English | ||||||
Publication Type: | Research article | ||||||
Article category: | Scientific peer reviewed | ||||||
Version: | Published version | ||||||
Copyright: | Creative Commons: Attribution 4.0 | ||||||
Full Text Status: | Public | ||||||
Subjects: | (A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Cell Biology (A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Botany | ||||||
Keywords: | arabidopsis, endodermis, casparian strip, microdomains, network, neprosin, A, thaliana | ||||||
URN:NBN: | urn:nbn:se:slu:epsilon-p-115960 | ||||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-115960 | ||||||
Additional ID: |
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ID Code: | 27131 | ||||||
Faculty: | S - Faculty of Forest Sciences | ||||||
Department: | (S) > Dept. of Forest Genetics and Plant Physiology | ||||||
Deposited By: | SLUpub Connector | ||||||
Deposited On: | 17 Feb 2022 18:25 | ||||||
Metadata Last Modified: | 18 Feb 2022 06:01 |
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