Home About Browse Search
Svenska


CASP microdomain formation requires cross cell wall stabilization of domains and non-cell autonomous action of LOTR1

Kolbeck, Andreas and Marhavy, Peter and De Bellis, Damien and Li, Baohai and Kamiya, Takehiro and Fujiwara, Toru and Kalmbach, Lothar and Geldner, Niko (2022). CASP microdomain formation requires cross cell wall stabilization of domains and non-cell autonomous action of LOTR1. eLife. 11 , e69602
[Research article]

[img] PDF
7MB

Abstract

Efficient uptake of nutrients in both animal and plant cells requires tissue-spanning diffusion barriers separating inner tissues from the outer lumen/soil. However, we poorly understand how such contiguous three-dimensional superstructures are formed in plants. Here, we show that correct establishment of the plant Casparian Strip (CS) network relies on local neighbor communication. We show that positioning of Casparian Strip membrane domains (CSDs) is tightly coordinated between neighbors in wild-type and that restriction of domain formation involves the putative extracellular protease LOTR1. Impaired domain restriction in lotr1 leads to fully functional CSDs at ectopic positions, forming 'half strips'. LOTR1 action in the endodermis requires its expression in the stele. LOTR1 endodermal expression cannot complement, while cortex expression causes a dominant-negative phenotype. Our findings establish LOTR1 as a crucial player in CSD positioning acting in a directional, non-cell-autonomous manner to restrict and coordinate CS positioning.

Authors/Creators:Kolbeck, Andreas and Marhavy, Peter and De Bellis, Damien and Li, Baohai and Kamiya, Takehiro and Fujiwara, Toru and Kalmbach, Lothar and Geldner, Niko
Title:CASP microdomain formation requires cross cell wall stabilization of domains and non-cell autonomous action of LOTR1
Series Name/Journal:eLife
Year of publishing :2022
Volume:11
Article number:e69602
Number of Pages:21
Publisher:eLIFE SCIENCES PUBL LTD
ISSN:2050-084X
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Cell Biology
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Botany
Keywords:arabidopsis, endodermis, casparian strip, microdomains, network, neprosin, A, thaliana
URN:NBN:urn:nbn:se:slu:epsilon-p-115960
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-115960
Additional ID:
Type of IDID
DOI10.7554/eLife.69602.sa2
Web of Science (WoS)000751409500001
ID Code:27131
Faculty:S - Faculty of Forest Sciences
Department:(S) > Dept. of Forest Genetics and Plant Physiology
Deposited By: SLUpub Connector
Deposited On:17 Feb 2022 18:25
Metadata Last Modified:18 Feb 2022 06:01

Repository Staff Only: item control page

Downloads

Downloads per year (since September 2012)

View more statistics

Downloads
Hits