Kalyandurg, Pruthvi Balachandra and Sundararajan, Poorva and Dubey, Mukesh and Ghadamgahi, Farideh and Zahid, Muhammad Awais and Whisson, Stephen C. and Vetukuri, Ramesh
(2021).
Spray-Induced Gene Silencing as a Potential Tool to Control Potato Late Blight Disease.
Phytopathology. 111
:12
, 2168-2175
[Research article]
![]() |
PDF
1MB |
Abstract
Phytophthora infestans causes late blight disease on potato and tomato and is currently controlled by resistant cultivars or intensive fungicide spraying. Here, we investigated an alternative means for late blight control by spraying potato leaves with double-stranded RNAs (dsRNA) that target the P. infestans genes essential for infection. First, we showed that the sporangia of P. infestans expressing green fluorescent protein (GFP) can take up in vitro synthesized dsRNAs homologous to GFP directly from their surroundings, including leaves, which led to the reduced relative expression of GFP. We further demonstrate the potential of spray-induced gene silencing (SIGS) in controlling potato late blight disease by targeting developmentally important genes in P. infestans such as guanine-nucleotide binding protein β-subunit (PiGPB1), haustorial membrane protein (PiHmp1), cutinase (PiCut3), and endo-1,3(4)-β-glucanase (PiEndo3). Our results demonstrate that SIGS can potentially be used to mitigate potato late blight; however, the degree of disease control is dependent on the selection of the target genes.
Authors/Creators: | Kalyandurg, Pruthvi Balachandra and Sundararajan, Poorva and Dubey, Mukesh and Ghadamgahi, Farideh and Zahid, Muhammad Awais and Whisson, Stephen C. and Vetukuri, Ramesh | ||||
---|---|---|---|---|---|
Title: | Spray-Induced Gene Silencing as a Potential Tool to Control Potato Late Blight Disease | ||||
Series Name/Journal: | Phytopathology | ||||
Year of publishing : | 2021 | ||||
Volume: | 111 | ||||
Number: | 12 | ||||
Page range: | 2168-2175 | ||||
Number of Pages: | 8 | ||||
ISSN: | 0031-949X | ||||
Language: | English | ||||
Publication Type: | Research article | ||||
Article category: | Scientific peer reviewed | ||||
Version: | Published version | ||||
Copyright: | Creative Commons: Attribution-Noncommercial-No Derivative Works 4.0 | ||||
Full Text Status: | Public | ||||
Subjects: | (A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science | ||||
Keywords: | climate change, disease control, host parasite interactions, oomycetes, pest management | ||||
URN:NBN: | urn:nbn:se:slu:epsilon-p-114915 | ||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-114915 | ||||
Additional ID: |
| ||||
ID Code: | 27140 | ||||
Faculty: | NJ - Fakulteten för naturresurser och jordbruksvetenskap LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap | ||||
Department: | (NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231) (LTJ, LTV) > Department of Plant Breeding (from 130101) (NL, NJ) > Dept. of Forest Mycology and Plant Pathology (S) > Dept. of Forest Mycology and Plant Pathology (LTJ, LTV) > Department of Plant Protection Biology | ||||
Deposited By: | SLUpub Connector | ||||
Deposited On: | 18 Feb 2022 11:25 | ||||
Metadata Last Modified: | 18 Feb 2022 11:31 |
Repository Staff Only: item control page