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Spray-Induced Gene Silencing as a Potential Tool to Control Potato Late Blight Disease

Kalyandurg, Pruthvi Balachandra and Sundararajan, Poorva and Dubey, Mukesh and Ghadamgahi, Farideh and Zahid, Muhammad Awais and Whisson, Stephen C. and Vetukuri, Ramesh (2021). Spray-Induced Gene Silencing as a Potential Tool to Control Potato Late Blight Disease. Phytopathology. 111 :12 , 2168-2175
[Research article]

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Abstract

Phytophthora infestans causes late blight disease on potato and tomato and is currently controlled by resistant cultivars or intensive fungicide spraying. Here, we investigated an alternative means for late blight control by spraying potato leaves with double-stranded RNAs (dsRNA) that target the P. infestans genes essential for infection. First, we showed that the sporangia of P. infestans expressing green fluorescent protein (GFP) can take up in vitro synthesized dsRNAs homologous to GFP directly from their surroundings, including leaves, which led to the reduced relative expression of GFP. We further demonstrate the potential of spray-induced gene silencing (SIGS) in controlling potato late blight disease by targeting developmentally important genes in P. infestans such as guanine-nucleotide binding protein β-subunit (PiGPB1), haustorial membrane protein (PiHmp1), cutinase (PiCut3), and endo-1,3(4)-β-glucanase (PiEndo3). Our results demonstrate that SIGS can potentially be used to mitigate potato late blight; however, the degree of disease control is dependent on the selection of the target genes.

Authors/Creators:Kalyandurg, Pruthvi Balachandra and Sundararajan, Poorva and Dubey, Mukesh and Ghadamgahi, Farideh and Zahid, Muhammad Awais and Whisson, Stephen C. and Vetukuri, Ramesh
Title:Spray-Induced Gene Silencing as a Potential Tool to Control Potato Late Blight Disease
Series Name/Journal:Phytopathology
Year of publishing :2021
Volume:111
Number:12
Page range:2168-2175
Number of Pages:8
ISSN:0031-949X
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution-Noncommercial-No Derivative Works 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science
Keywords:climate change, disease control, host parasite interactions, oomycetes, pest management
URN:NBN:urn:nbn:se:slu:epsilon-p-114915
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-114915
Additional ID:
Type of IDID
DOI10.1094/PHYTO-02-21-0054-SC
ID Code:27140
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231)
(LTJ, LTV) > Department of Plant Breeding (from 130101)
(NL, NJ) > Dept. of Forest Mycology and Plant Pathology
(S) > Dept. of Forest Mycology and Plant Pathology

(LTJ, LTV) > Department of Plant Protection Biology
Deposited By: SLUpub Connector
Deposited On:18 Feb 2022 11:25
Metadata Last Modified:18 Feb 2022 11:31

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