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An in vitro-based hazard assessment of liquid smoke food flavourings

Selin, Erica and Mandava, Geeta and Vilcu, Alexandra and Oskarsson, Agneta and Lundqvist, Johan (2022). An in vitro-based hazard assessment of liquid smoke food flavourings. Archives of Toxicology. 96 :2 , 601-611
[Research article]

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Abstract

Liquid smoke products are widely used as a food additive to create a desired smoke flavour. These products may contain hazardous chemicals generated during the wood-burning process. However, the toxic effects of these types of hazardous chemicals constituting in the commercially available products are largely unknown. Therefore, a test battery of cell-based in vitro methods, covering different modes of actions of high relevance to human health, was applied to study liquid smoke products. Ten liquid smoke flavourings were tested as non-extracted and extracted. To assess the potential drivers of toxicity, we used two different solvents. The battery of in vitro methods covered estrogenicity, androgenicity, oxidative stress, aryl hydrocarbon receptor activity and genotoxicity. The non-extracted samples were tested at concentrations 0.002 to 1 mu L liquid smoke flavouring/mL culture medium, while extracted samples were tested from 0.003 to 200 mu L/mL. Genotoxicity was observed for nearly all non-extracted and all hexane-extracted samples, in which the former had higher potency. No genotoxicity was observed for ethyl acetate-extracted samples. Oxidative stress was activated by almost all extracted and non-extracted samples, while approximately half of the samples had aryl hydrocarbon receptor and estrogen receptor activities. This study used effect-based methods to evaluate the complex mixtures of liquid smoke flavourings. The increased bioactivities seen upon extractions indicate that non-polar chemicals are driving the genotoxicity, while polar substances are increasing oxidative stress and cytotoxic responses. The differences in responses indicate that non-extracted products contain chemicals that are able to antagonize toxic effects, and upon extraction, the protective substances are lost.

Authors/Creators:Selin, Erica and Mandava, Geeta and Vilcu, Alexandra and Oskarsson, Agneta and Lundqvist, Johan
Title:An in vitro-based hazard assessment of liquid smoke food flavourings
Series Name/Journal:Archives of Toxicology
Year of publishing :2022
Volume:96
Number:2
Page range:601-611
Number of Pages:11
Publisher:SPRINGER HEIDELBERG
ISSN:0340-5761
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Food Science
Keywords:Smoke flavouring, Commercial liquid smoke flavouring, Food additives, Bioassays, Bioanalytical tool, Effect-based methods
URN:NBN:urn:nbn:se:slu:epsilon-p-114531
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-114531
Additional ID:
Type of IDID
DOI10.1007/s00204-021-03190-1
Web of Science (WoS)000720597400001
ID Code:27150
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Department of Biomedical Science and Veterinary Public Health
Deposited By: SLUpub Connector
Deposited On:21 Feb 2022 10:25
Metadata Last Modified:21 Feb 2022 10:31

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