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Progesterone differentially affects the transcriptomic profiles of cow endometrial cell types

Pereira, Goncalo and Guo, Yongzhi and Silva, Elisabete and Bevilacqua, Claudia and Charpigny, Gilles and Lopes-da-Costa, Luis and Humblot, Patrice (2022). Progesterone differentially affects the transcriptomic profiles of cow endometrial cell types. BMC Genomics. 23 :1 , 82
[Research article]

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Abstract

Background The endometrium is a heterogeneous tissue composed of luminal epithelial (LE), glandular epithelial (GE), and stromal cells (ST), experiencing progesterone regulated dynamic changes during the estrous cycle. In the cow, this regulation at the transcriptomic level was only evaluated in the whole tissue. This study describes specific gene expression in the three types of cells isolated from endometrial biopsies following laser capture microdissection and the transcriptome changes induced by progesterone in GE and ST cells. Results Endometrial LE, GE, and ST cells show specific transcriptomic profiles. Most of the differentially expressed genes (DEGs) in response to progesterone are cell type-specific (96%). Genes involved in cell cycle and nuclear division are under-expressed in the presence of progesterone in GE, highlighting the anti-proliferative action of progesterone in epithelial cells. Elevated progesterone concentrations are also associated with the under-expression of estrogen receptor 1 (ESR1) in GE and oxytocin receptor (OXTR) in GE and ST cells. In ST cells, transcription factors such as SOX17 and FOXA2, known to regulate uterine epithelial-stromal cross-talk conveying to endometrial receptivity, are over-expressed under progesterone influence. Conclusions The results from this study show that progesterone regulates endometrial function in a cell type-specific way, which is independent of the expression of its main receptor PGR. These novel insights into uterine physiology present the cell compartment as the physiological unit rather than the whole tissue.

Authors/Creators:Pereira, Goncalo and Guo, Yongzhi and Silva, Elisabete and Bevilacqua, Claudia and Charpigny, Gilles and Lopes-da-Costa, Luis and Humblot, Patrice
Title:Progesterone differentially affects the transcriptomic profiles of cow endometrial cell types
Series Name/Journal:BMC Genomics
Year of publishing :2022
Volume:23
Number:1
Article number:82
Number of Pages:19
Publisher:BMC
ISSN:1471-2164
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
Keywords:Transcriptome, Progesterone, Endometrium, LCM, RNA-seq
URN:NBN:urn:nbn:se:slu:epsilon-p-116095
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-116095
Additional ID:
Type of IDID
DOI10.1186/s12864-022-08323-z
Web of Science (WoS)000749206000002
ID Code:27175
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
Deposited By: SLUpub Connector
Deposited On:22 Feb 2022 14:25
Metadata Last Modified:22 Feb 2022 14:31

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