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Genome-wide analysis of the response to ivermectin treatment by a Swedish field population of Haemonchus contortus

Baltrusis, Paulius and Doyle, Stephen R. and Halvarsson, Peter and Höglund, Johan (2022). Genome-wide analysis of the response to ivermectin treatment by a Swedish field population of Haemonchus contortus. International Journal for Parasitology: Drugs and Drug Resistance. 18 , 12-19
[Research article]

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Abstract

Haemonchus contortus is a pathogenic gastrointestinal nematode of small ruminants and, in part due to its capacity to develop resistance to drugs, contributes to significant losses in the animal production sector worldwide. Despite decades of research, comparatively little is known about the specific mechanism(s) driving resistance to drugs such as ivermectin in this species. Here we describe a genome-wide approach to detect evidence of selection by ivermectin treatment in a field population of H. contortus from Sweden, using parasites sampled from the same animals before and seven days after ivermectin exposure followed by whole-genome sequencing. Despite an 89% reduction in parasites recovered after treatment measured by the fecal egg count reduction test, the surviving population was highly genetically similar to the population before treatment, suggesting that resistance has likely evolved over time and that resistance alleles are present on diverse haplotypes. Pairwise gene and SNP frequency comparisons indicated the highest degree of differentiation was found at the terminal end of chromosome 4, whereas the most striking difference in nucleotide diversity was observed in a region on chromosome 5 previously reported to harbor a major quantitative trait locus involved in ivermectin resistance. These data provide novel insight into the genome-wide effect of ivermectin selection in a field population as well as confirm the importance of the previously established quantitative trait locus in the development of resistance to ivermectin.

Authors/Creators:Baltrusis, Paulius and Doyle, Stephen R. and Halvarsson, Peter and Höglund, Johan
Title:Genome-wide analysis of the response to ivermectin treatment by a Swedish field population of Haemonchus contortus
Series Name/Journal:International Journal for Parasitology: Drugs and Drug Resistance
Year of publishing :2022
Volume:18
Page range:12-19
Number of Pages:8
Publisher:ELSEVIER SCI LTD
ISSN:2211-3207
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Pathobiology
Keywords:Haemonchus contortus, Ivermectin, Whole-genome sequencing, Anthelmintic resistance, Pool-seq
URN:NBN:urn:nbn:se:slu:epsilon-p-116100
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-116100
Additional ID:
Type of IDID
DOI10.1016/j.ijpddr.2021.12.002
Web of Science (WoS)000746499100001
ID Code:27177
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Department of Biomedical Science and Veterinary Public Health
Deposited By: SLUpub Connector
Deposited On:22 Feb 2022 14:25
Metadata Last Modified:22 Feb 2022 14:31

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