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The Physcomitrium patens egg cell expresses several distinct epigenetic components and utilizes homologues of BONOBO genes for cell specification

Sanchez, Vera Victoria and Landberg, Katarina and Lopez, Obando Mauricio and Thelander, Mattias and Lagercrantz, Ulf and Muñoz, Rafael and Schmidt, Anja and Grossniklaus, Ueli and Sundberg, Eva (2022). The Physcomitrium patens egg cell expresses several distinct epigenetic components and utilizes homologues of BONOBO genes for cell specification. New Phytologist. 233 :6 , 2614-2628
[Research article]

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Abstract

Although land plant germ cells have received much attention, knowledge about their specification is still limited. We thus identified transcripts enriched in egg cells of the bryophyte model species Physcomitrium patens, compared the results with angiosperm egg cells, and selected important candidate genes for functional analysis. We used laser-assisted microdissection to perform a cell-type-specific transcriptome analysis on egg cells for comparison with available expression profiles of vegetative tissues and male reproductive organs. We made reporter lines and knockout mutants of the two BONOBO (PbBNB) genes and studied their role in reproduction. We observed an overlap in gene activity between bryophyte and angiosperm egg cells, but also clear differences. Strikingly, several processes that are male-germline specific in Arabidopsis are active in the P. patens egg cell. Among those were the moss PbBNB genes, which control proliferation and identity of both female and male germlines. Pathways shared between male and female germlines were most likely present in the common ancestors of land plants, besides sex-specifying factors. A set of genes may also be involved in the switches between the diploid and haploid moss generations. Nonangiosperm gene networks also contribute to the specification of the P. patens egg cell.

Authors/Creators:Sanchez, Vera Victoria and Landberg, Katarina and Lopez, Obando Mauricio and Thelander, Mattias and Lagercrantz, Ulf and Muñoz, Rafael and Schmidt, Anja and Grossniklaus, Ueli and Sundberg, Eva
Title:The Physcomitrium patens egg cell expresses several distinct epigenetic components and utilizes homologues of BONOBO genes for cell specification
Series Name/Journal:New Phytologist
Year of publishing :2022
Volume:233
Number:6
Page range:2614-2628
Number of Pages:15
Publisher:WILEY
ISSN:0028-646X
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution-Noncommercial-No Derivative Works 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Cell Biology
Keywords:BONOBO, bryophytes, egg cell, gametes, moss, Physcomitrium patens, transcriptome
URN:NBN:urn:nbn:se:slu:epsilon-p-116176
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-116176
Additional ID:
Type of IDID
DOI10.1111/nph.17938
Web of Science (WoS)000744963500001
ID Code:27187
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231)
Deposited By: SLUpub Connector
Deposited On:23 Feb 2022 13:25
Metadata Last Modified:23 Feb 2022 13:31

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