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Practical Method for Freezing Buck Semen

Morrell, Jane and Malaluang, Pongpreecha and Ntallaris, Theodoros and Johannisson, Anders (2022). Practical Method for Freezing Buck Semen. Animals. 12 :3 , 352
[Research article]

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Abstract

Although several protocols for cryopreserving buck semen are described in the literature, they differ widely in factors such as season and method of semen collection, extender and sperm concentration. Therefore, choosing a protocol that is suitable for a particular on-farm situation can be problematic. In the present study, semen was collected by artificial vagina from seven bucks on a farm located approximately 90 minutes’ drive away from the laboratory, about 6 weeks before the start of the goat breeding season. The semen was immediately extended in warm semen extender containing soy lecithin and was placed in an insulated box with a cold pack for up to 4 h, during semen collection from the remaining bucks and subsequent transport to the laboratory. Following centrifugation at 4 °C and resuspension in the soy lecithin extender to a sperm concentration of 800 × 106 spermatozoa/mL, 0.25 mL plastic straws were filled and frozen in racks 4 cm above the surface of liquid nitrogen. This simple protocol resulted in an acceptable post-thaw quality for all seven bucks, with a mean post-thaw motility of 55 ± 21% and mean fragmented chromatin of 3.27 ± 1.39%. Normal sperm morphology was >90% in all ejaculates. The semen was sent to a gamete bank for long-term storage.

Authors/Creators:Morrell, Jane and Malaluang, Pongpreecha and Ntallaris, Theodoros and Johannisson, Anders
Title:Practical Method for Freezing Buck Semen
Series Name/Journal:Animals
Year of publishing :2022
Volume:12
Number:3
Article number:352
Number of Pages:10
ISSN:2076-2615
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 402 Animal and Dairy Science > Animal and Dairy Science.
Keywords:goat sperm cryopreservation, removal of seminal plasma, soy lecithin semen extender, plasma membrane integrity, chromatin integrity
URN:NBN:urn:nbn:se:slu:epsilon-p-116221
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-116221
Additional ID:
Type of IDID
DOI10.3390/ani12030352
ID Code:27230
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
Deposited By: SLUpub Connector
Deposited On:28 Feb 2022 14:25
Metadata Last Modified:28 Feb 2022 14:31

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