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Bypassing reproductive barriers in hybrid seeds using chemically induced epimutagenesis

Huc, Jonathan and Dziasek, Katarzyna and Pachamuthu, Kannan and Woh, Tristan and Köhler, Claudia and Borges, Filipe (2021). Bypassing reproductive barriers in hybrid seeds using chemically induced epimutagenesis. Plant Cell. 34 :3 , 989–1001
[Research article]

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Abstract

The triploid block, which prevents interploidy hybridizations in flowering plants, is characterized by a failure in endosperm development, arrest in embryogenesis, and seed collapse. Many genetic components of triploid seed lethality have been successfully identified in the model plant Arabidopsis thaliana, most notably the paternally expressed genes (PEGs), which are upregulated in tetraploid endosperm with paternal excess. Previous studies have shown that the paternal epigenome is a key determinant of the triploid block response, as the loss of DNA methylation in diploid pollen suppresses the triploid block almost completely. Here, we demonstrate that triploid seed collapse is bypassed in Arabidopsis plants treated with the DNA methyltransferase inhibitor 5-Azacytidine during seed germination and early growth. We identified strong suppressor lines showing stable transgenerational inheritance of hypomethylation in the CG context, as well as normalized expression of PEGs in triploid seeds. Importantly, differentially methylated loci segregate in the progeny of “epimutagenized” plants, which may allow epialleles involved in the triploid block response to be identified in future studies. Finally, we demonstrate that chemically induced epimutagenesis facilitates hybridization between different Capsella species, thus potentially emerging as a strategy for producing triploids and interspecific hybrids with high agronomic interest.

Authors/Creators:Huc, Jonathan and Dziasek, Katarzyna and Pachamuthu, Kannan and Woh, Tristan and Köhler, Claudia and Borges, Filipe
Title:Bypassing reproductive barriers in hybrid seeds using chemically induced epimutagenesis
Series Name/Journal:Plant Cell
Year of publishing :2021
Volume:34
Number:3
Page range:989–1001
Number of Pages:13
ISSN:1040-4651
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Plant Biotechnology
URN:NBN:urn:nbn:se:slu:epsilon-p-114585
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-114585
Additional ID:
Type of IDID
DOI10.1093/plcell/koab284
ID Code:27267
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231)
Deposited By: SLUpub Connector
Deposited On:07 Mar 2022 16:25
Metadata Last Modified:07 Mar 2022 16:31

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