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FERONIA and microtubules independently contribute to mechanical integrity in the Arabidopsis shoot

Malivert, Alice and Erguvan, Ozer and Chevallier, Antoine and Dehem, Antoine and Friaud, Rodrigue and Liu, Mengying and Martin, Marjolaine and Peyraud, Theophile and Hamant, Olivier and Verger, Stephane (2021). FERONIA and microtubules independently contribute to mechanical integrity in the Arabidopsis shoot. PLoS Biology. 19 :11 , e3001454
[Research article]

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Abstract

To survive, cells must constantly resist mechanical stress. In plants, this involves the reinforcement of cell walls, notably through microtubule-dependent cellulose deposition. How wall sensing might contribute to this response is unknown. Here, we tested whether the microtubule response to stress acts downstream of known wall sensors. Using a multistep screen with 11 mutant lines, we identify FERONIA (FER) as the primary candidate for the cell's response to stress in the shoot. However, this does not imply that FER acts upstream of the microtubule response to stress. In fact, when performing mechanical perturbations, we instead show that the expected microtubule response to stress does not require FER. We reveal that the feronia phenotype can be partially rescued by reducing tensile stress levels. Conversely, in the absence of both microtubules and FER, cells appear to swell and burst. Altogether, this shows that the microtubule response to stress acts as an independent pathway to resist stress, in parallel to FER. We propose that both pathways are required to maintain the mechanical integrity of plant cells.

Authors/Creators:Malivert, Alice and Erguvan, Ozer and Chevallier, Antoine and Dehem, Antoine and Friaud, Rodrigue and Liu, Mengying and Martin, Marjolaine and Peyraud, Theophile and Hamant, Olivier and Verger, Stephane
Title:FERONIA and microtubules independently contribute to mechanical integrity in the Arabidopsis shoot
Series Name/Journal:PLoS Biology
Year of publishing :2021
Volume:19
Number:11
Article number:e3001454
Number of Pages:22
Publisher:PUBLIC LIBRARY SCIENCE
ISSN:1544-9173
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 3 Medical and Health Sciences > 304 Medical Biotechnology > Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
URN:NBN:urn:nbn:se:slu:epsilon-p-116315
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-116315
Additional ID:
Type of IDID
DOI10.1371/journal.pbio.3001454
Web of Science (WoS)000750903600005
ID Code:27300
Deposited By: SLUpub Connector
Deposited On:14 Mar 2022 08:25
Metadata Last Modified:14 Mar 2022 08:31

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