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Discovery of a Novel Leaf Rust (Puccinia recondita) Resistance Gene in Rye (Secale cereale L.) Using Association Genomics

Vendelbo, Nikolaj Meisner and Mahmood, Khalid and Sarup, Pernille and Hovmoller, Mogens S. and Justesen, Annemarie Fejer and Kristensen, Peter Skov and Orabi, Jihad and Jahoor, Ahmed (2022). Discovery of a Novel Leaf Rust (Puccinia recondita) Resistance Gene in Rye (Secale cereale L.) Using Association Genomics. Cells. 11 :1 , 64
[Research article]

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Abstract

Leaf rust constitutes one of the most important foliar diseases in rye (Secale cereale L.). To discover new sources of resistance, we phenotyped 180 lines belonging to a less well-characterized Gulzow germplasm at three field trial locations in Denmark and Northern Germany in 2018 and 2019. We observed lines with high leaf rust resistance efficacy at all locations in both years. A genome-wide association study using 261,406 informative single-nucleotide polymorphisms revealed two genomic regions associated with resistance on chromosome arms 1RS and 7RS, respectively. The most resistance-associated marker on chromosome arm 1RS physically co-localized with molecular markers delimiting Pr3. In the reference genomes Lo7 and Weining, the genomic region associated with resistance on chromosome arm 7RS contained a large number of nucleotide-binding leucine-rich repeat (NLR) genes. Residing in close proximity to the most resistance-associated marker, we identified a cluster of NLRs exhibiting close protein sequence similarity with the wheat leaf rust Lr1 gene situated on chromosome arm 5DL in wheat, which is syntenic to chromosome arm 7RS in rye. Due to the close proximity to the most resistance-associated marker, our findings suggest that the considered leaf rust R gene, provisionally denoted Pr6, could be a Lr1 ortholog in rye.

Authors/Creators:Vendelbo, Nikolaj Meisner and Mahmood, Khalid and Sarup, Pernille and Hovmoller, Mogens S. and Justesen, Annemarie Fejer and Kristensen, Peter Skov and Orabi, Jihad and Jahoor, Ahmed
Title:Discovery of a Novel Leaf Rust (Puccinia recondita) Resistance Gene in Rye (Secale cereale L.) Using Association Genomics
Series Name/Journal:Cells
Year of publishing :2022
Volume:11
Number:1
Article number:64
Number of Pages:19
Publisher:MDPI
ISSN:2073-4409
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science
Keywords:hybrid breeding, Gulzow germplasm, field trial, disease progression, 600K high-density SNP array, nucleotide-binding leucine-rich repeat (NLR), NB-ARC domain, phylogenetic analysis, Lr1-like disease resistance protein
URN:NBN:urn:nbn:se:slu:epsilon-p-116257
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-116257
Additional ID:
Type of IDID
DOI10.3390/cells11010064
Web of Science (WoS)000751048800001
ID Code:27316
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Breeding (from 130101)
Deposited By: SLUpub Connector
Deposited On:15 Mar 2022 09:25
Metadata Last Modified:15 Mar 2022 09:31

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