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Transcriptome Analysis of an Aedes albopictus Cell Line Single- and Dual-Infected with Lammi Virus and WNV

Öhlund, Pontus and Delhomme, Nicolas and Hayer, Juliette and Hesson, Jenny C. and Blomström, Anne-Lie (2022). Transcriptome Analysis of an Aedes albopictus Cell Line Single- and Dual-Infected with Lammi Virus and WNV. International Journal of Molecular Sciences. 23 :2 , 875
[Research article]

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Abstract

Understanding the flavivirus infection process in mosquito hosts is important and fundamental in the search for novel control strategies that target the mosquitoes' ability to carry and transmit pathogenic arboviruses. A group of viruses known as insect-specific viruses (ISVs) has been shown to interfere with the infection and replication of a secondary arbovirus infection in mosquitoes and mosquito-derived cell lines. However, the molecular mechanisms behind this interference are unknown. Therefore, in the present study, we infected the Aedes albopictus cell line U4.4 with either the West Nile virus (WNV), the insect-specific Lammi virus (LamV) or an infection scheme whereby cells were pre-infected with LamV 24 h prior to WNV challenge. The qPCR analysis showed that the dual-infected U4.4 cells had a reduced number of WNV RNA copies compared to WNV-only infected cells. The transcriptome profiles of the different infection groups showed a variety of genes with altered expression. WNV-infected cells had an up-regulation of a broad range of immune-related genes, while in LamV-infected cells, many genes related to stress, such as different heat-shock proteins, were up-regulated. The transcriptome profile of the dual-infected cells was a mix of up- and down-regulated genes triggered by both viruses. Furthermore, we observed an up-regulation of signal peptidase complex (SPC) proteins in all infection groups. These SPC proteins have shown importance for flavivirus assembly and secretion and could be potential targets for gene modification in strategies for the interruption of flavivirus transmission by mosquitoes.

Authors/Creators:Öhlund, Pontus and Delhomme, Nicolas and Hayer, Juliette and Hesson, Jenny C. and Blomström, Anne-Lie
Title:Transcriptome Analysis of an Aedes albopictus Cell Line Single- and Dual-Infected with Lammi Virus and WNV
Series Name/Journal:International Journal of Molecular Sciences
Year of publishing :2022
Volume:23
Number:2
Article number:875
Number of Pages:17
Publisher:MDPI
ISSN:1661-6596
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology
Keywords:insect-specific flaviviruses, West Nile virus, Aedes albopictus, transcriptome, viral interference
URN:NBN:urn:nbn:se:slu:epsilon-p-116406
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-116406
Additional ID:
Type of IDID
DOI10.3390/ijms23020875
Web of Science (WoS)000758485600001
ID Code:27340
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
S - Faculty of Forest Sciences
Department:(VH) > Department of Biomedical Science and Veterinary Public Health
(S) > Dept. of Forest Genetics and Plant Physiology
(VH) > Dept. of Animal Breeding and Genetics
Deposited By: SLUpub Connector
Deposited On:18 Mar 2022 11:25
Metadata Last Modified:18 Mar 2022 11:31

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