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Analysis of the transcriptome of bovine endometrial cells isolated by laser micro-dissection (1): specific signatures of stromal, glandular and luminal epithelial cells

Chankeaw, Wiruntita and Lignier, Sandra and Richard, Christophe and Ntallaris, Theodoros and Raliou, Mariam and Guo, Yongzhi and Plassard, Damien and Bevilacqua, Claudia and Sandra, Olivier and Andersson, Göran and Humblot, Patrice and Charpigny, Gilles (2021). Analysis of the transcriptome of bovine endometrial cells isolated by laser micro-dissection (1): specific signatures of stromal, glandular and luminal epithelial cells. BMC Genomics. 22 :1 , 451
[Research article]

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Abstract

Background A number of studies have examined mRNA expression profiles of bovine endometrium at estrus and around the peri-implantation period of pregnancy. However, to date, these studies have been performed on the whole endometrium which is a complex tissue. Consequently, the knowledge of cell-specific gene expression, when analysis performed with whole endometrium, is still weak and obviously limits the relevance of the results of gene expression studies. Thus, the aim of this study was to characterize specific transcriptome of the three main cell-types of the bovine endometrium at day-15 of the estrus cycle. Results In the RNA-Seq analysis, the number of expressed genes detected over 10 transcripts per million was 6622, 7814 and 8242 for LE, GE and ST respectively. ST expressed exclusively 1236 genes while only 551 transcripts were specific to the GE and 330 specific to LE. For ST, over-represented biological processes included many regulation processes and response to stimulus, cell communication and cell adhesion, extracellular matrix organization as well as developmental process. For GE, cilium organization, cilium movement, protein localization to cilium and microtubule-based process were the only four main biological processes enriched. For LE, over-represented biological processes were enzyme linked receptor protein signaling pathway, cell-substrate adhesion and circulatory system process. Conclusion The data show that each endometrial cell-type has a distinct molecular signature and provide a significantly improved overview on the biological process supported by specific cell-types. The most interesting result is that stromal cells express more genes than the two epithelial types and are associated with a greater number of pathways and ontology terms.

Authors/Creators:Chankeaw, Wiruntita and Lignier, Sandra and Richard, Christophe and Ntallaris, Theodoros and Raliou, Mariam and Guo, Yongzhi and Plassard, Damien and Bevilacqua, Claudia and Sandra, Olivier and Andersson, Göran and Humblot, Patrice and Charpigny, Gilles
Title:Analysis of the transcriptome of bovine endometrial cells isolated by laser micro-dissection (1): specific signatures of stromal, glandular and luminal epithelial cells
Series Name/Journal:BMC Genomics
Year of publishing :2021
Volume:22
Number:1
Article number:451
Number of Pages:16
Publisher:BMC
ISSN:1471-2164
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
Keywords:Cow, Endometrium, Cell type-specific, Transcriptome, LCM, RNA-seq
URN:NBN:urn:nbn:se:slu:epsilon-p-112746
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-112746
Additional ID:
Type of IDID
DOI10.1186/s12864-021-07712-0
Web of Science (WoS)000664630000001
ID Code:27419
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
(VH) > Dept. of Animal Breeding and Genetics
Deposited By: SLUpub Connector
Deposited On:23 Mar 2022 10:25
Metadata Last Modified:23 Mar 2022 10:31

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