Home About Browse Search
Svenska


Morphological and Comparative Transcriptome Analysis of Three Species of Five-Needle Pines: Insights Into Phenotypic Evolution and Phylogeny

Li, Xiang and Cai, Kewei and Zhao, Qiushuang and Li, Hanxi and Wang, Xuelai and Tigabu, Mulualem and Sederoff, Ronald and Ma, Wenjun and Zhao, Xiyang (2022). Morphological and Comparative Transcriptome Analysis of Three Species of Five-Needle Pines: Insights Into Phenotypic Evolution and Phylogeny. Frontiers in Plant Science. 13 , 795631
[Research article]

[img] PDF
4MB

Abstract

Pinus koraiensis, Pinus sibirica, and Pinus pumila are the major five-needle pines in northeast China, with substantial economic and ecological values. The phenotypic variation, environmental adaptability and evolutionary relationships of these three five-needle pines remain largely undecided. It is therefore important to study their genetic differentiation and evolutionary history. To obtain more genetic information, the needle transcriptomes of the three five-needle pines were sequenced and assembled. To explore the relationship of sequence information and adaptation to a high mountain environment, data on needle morphological traits [needle length (NL), needle width (NW), needle thickness (NT), and fascicle width (FW)] and 19 climatic variables describing the patterns and intensity of temperature and precipitation at six natural populations were recorded. Geographic coordinates of altitude, latitude, and longitude were also obtained. The needle morphological data was combined with transcriptome information, location, and climate data, for a comparative analysis of the three five-needle pines. We found significant differences for needle traits among the populations of the three five-needle pine species. Transcriptome analysis showed that the phenotypic variation and environmental adaptation of the needles of P. koraiensis, P. sibirica, and P. pumila were related to photosynthesis, respiration, and metabolites. Analysis of orthologs from 11 Pinus species indicated a closer genetic relationship between P. koraiensis and P. sibirica compared to P. pumila. Our study lays a foundation for genetic improvement of these five-needle pines and provides insights into the adaptation and evolution of Pinus species.

Authors/Creators:Li, Xiang and Cai, Kewei and Zhao, Qiushuang and Li, Hanxi and Wang, Xuelai and Tigabu, Mulualem and Sederoff, Ronald and Ma, Wenjun and Zhao, Xiyang
Title:Morphological and Comparative Transcriptome Analysis of Three Species of Five-Needle Pines: Insights Into Phenotypic Evolution and Phylogeny
Series Name/Journal:Frontiers in Plant Science
Year of publishing :2022
Volume:13
Article number:795631
Number of Pages:14
Publisher:FRONTIERS MEDIA SA
ISSN:1664-462X
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Forest Science
Keywords:five-needle pines, Pinus koraiensis, Pinus sibirica, Pinus pumila, transcriptome sequencing, SSRs, Pinus phylogeny
URN:NBN:urn:nbn:se:slu:epsilon-p-116390
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-116390
Additional ID:
Type of IDID
DOI10.3389/fpls.2022.795631
Web of Science (WoS)000760785700001
ID Code:27487
Faculty:S - Faculty of Forest Sciences
Department:(S) > Southern Swedish Forest Research Centre
Deposited By: SLUpub Connector
Deposited On:31 Mar 2022 13:25
Metadata Last Modified:31 Mar 2022 13:31

Repository Staff Only: item control page

Downloads

Downloads per year (since September 2012)

View more statistics

Downloads
Hits