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Chemosensory Receptors in the Larval Maxilla of Papilio hospiton

Crava, Cristina M. and Bobkov, Yuriy V. and Sollai, Giorgia and Anfora, Gianfranco and Crnjar, Roberto and Cattaneo, Alberto Maria (2022). Chemosensory Receptors in the Larval Maxilla of Papilio hospiton. Frontiers in Ecology and Evolution. 9 , 795994
[Research article]

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Abstract

Among the butterflies of the genus Papilio (Lepidoptera: Papilionidae), Papilio hospiton (Gene) has a geographical distribution limited to the Mediterranean islands of Sardinia (Italy) and Corsica (France). This is mainly due to the host range that includes only a few plant species of Apiaceae and Rutaceae growing on these islands. In a previous electrophysiological investigation conducted on the maxillary gustatory system of larvae of P. hospiton and its closely phylogenetically related species Papilio machaon, a significantly higher spike activity was shown for the gustatory neurons of lateral and medial styloconic sensilla in P. hospiton when bitter compounds were tested. This effect was possibly correlated to the limited host choice range for P. hospiton. To shed light on the molecular aspects of this phenomenon, we investigated the expression pattern of sensory-related sequences by conducting a transcriptomic analysis from total RNA isolates of P. hospiton larval maxillae. We identified several transcripts that may be involved in taste (one gustatory receptor, one divergent ionotropic receptor, and several transient receptor potential channels, TRPs) as well as transcripts supporting an olfactory function for this appendage, including odorant receptors (ORs), antennal ionotropic receptors (A-IRs), sensory neuron membrane proteins (SNMPs), and odorant-binding proteins (OBPs). We used Human Embryonic Kidney (HEK293A) cells to heterologously express two of the identified receptors, PhospOR1 and PhospPain, together with their orthologs from P. machaon, for functional characterization. While our data suggest no activation of these two receptors by the ligands known so far to activate the electrophysiological response in larval maxillary neurons of Papilio species, nor temperature activation of both Papilio TRPA-channel Painless, they represent the first attempt in connecting neuronal activity with their molecular bases to unravel diet specialization between closely related Papilio species.

Authors/Creators:Crava, Cristina M. and Bobkov, Yuriy V. and Sollai, Giorgia and Anfora, Gianfranco and Crnjar, Roberto and Cattaneo, Alberto Maria
Title:Chemosensory Receptors in the Larval Maxilla of Papilio hospiton
Series Name/Journal:Frontiers in Ecology and Evolution
Year of publishing :2022
Volume:9
Article number:795994
Number of Pages:20
Publisher:FRONTIERS MEDIA SA
ISSN:2296-701X
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Zoology
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Ecology
Keywords:papilionid butterflies, larval maxilla, RNA-seq analysis, odorant receptors, transient receptor potential (TRP) channels, heterologous expression, human embryonic kidney cells
URN:NBN:urn:nbn:se:slu:epsilon-p-116391
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-116391
Additional ID:
Type of IDID
DOI10.3389/fevo.2021.795994
Web of Science (WoS)000759817700001
ID Code:27514
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Protection Biology
Deposited By: SLUpub Connector
Deposited On:07 Apr 2022 12:09
Metadata Last Modified:07 Apr 2022 12:11

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