Home About Browse Search
Svenska


Coincident Fluorescence-Burst Analysis of the Loading Yields of Exosome-Mimetic Nanovesicles with Fluorescently-Labeled Cargo Molecules

Sanaee, Maryam and Sandberg, Elin and Ronquist, Göran and Morrell, Jane and Widengren, Jerker and Gallo, Katia (2022). Coincident Fluorescence-Burst Analysis of the Loading Yields of Exosome-Mimetic Nanovesicles with Fluorescently-Labeled Cargo Molecules. Small. 18 :12 , 2106241
[Research article]

[img] PDF
1MB

Abstract

The possible targeting functionality and low immunogenicity of exosomes and exosome-like nanovesicles make them promising as drug-delivery carriers. To tap into this potential, accurate non-destructive methods to load them and characterize their contents are of utmost importance. However, the small size, polydispersity, and aggregation of nanovesicles in solution make quantitative characterizations of their loading particularly challenging. Here, an ad-hoc methodology is developed based on burst analysis of dual-color confocal fluorescence microscopy experiments, suited for quantitative characterizations of exosome-like nanovesicles and of their fluorescently-labeled loading. It is applied to study exosome-mimetic nanovesicles derived from animal extracellular-vesicles and human red blood cell detergent-resistant membranes, loaded with fluorescently-tagged dUTP cargo molecules. For both classes of nanovesicles, successful loading is proved and by dual-color coincident fluorescence burst analysis, size statistics and loading yields are retrieved and quantified. The procedure affords single-vesicle characterizations well-suited for the investigation of a variety of cargo molecules and biological nanovesicle combinations besides the proof-of-principle demonstrations of this study. The results highlight a powerful characterization tool essential for optimizing the loading process and for advanced engineering of biomimetic nanovesicles for therapeutic drug delivery.

Authors/Creators:Sanaee, Maryam and Sandberg, Elin and Ronquist, Göran and Morrell, Jane and Widengren, Jerker and Gallo, Katia
Title:Coincident Fluorescence-Burst Analysis of the Loading Yields of Exosome-Mimetic Nanovesicles with Fluorescently-Labeled Cargo Molecules
Series Name/Journal:Small
Year of publishing :2022
Volume:18
Number:12
Article number:2106241
Number of Pages:11
Publisher:WILEY-V C H VERLAG GMBH
ISSN:1613-6810
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution-Noncommercial-No Derivative Works 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 3 Medical and Health Sciences > 301 Basic Medicine > Pharmaceutical Sciences
Keywords:burst analysis, drug loading, extracellular nanovesicles, fluorescence correlation spectroscopy, single vesicle profiling
URN:NBN:urn:nbn:se:slu:epsilon-p-116094
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-116094
Additional ID:
Type of IDID
DOI10.1002/smll.202106241
Web of Science (WoS)000748617600001
ID Code:27529
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
Deposited By: SLUpub Connector
Deposited On:11 Apr 2022 18:25
Metadata Last Modified:12 Apr 2022 05:01

Repository Staff Only: item control page

Downloads

Downloads per year (since September 2012)

View more statistics

Downloads
Hits