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The miRNome function transitions from regulating developmental genes to transposable elements during pollen maturation

Oliver Velasco, Cecilia and Annacondia, María Luz and Wang, Zhenxing and Jullien, Pauline E. and Slotkin, R. Keith and Köhler, Claudia and Martinez Arias, German (2022). The miRNome function transitions from regulating developmental genes to transposable elements during pollen maturation. Plant Cell. 34 :2 , 784-801
[Research article]

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Abstract

Animal and plant microRNAs (miRNAs) are essential for the spatio-temporal regulation of development. Together with this role, plant miRNAs have been proposed to target transposable elements (TEs) and stimulate the production of epigenetically active small interfering RNAs. This activity is evident in the plant male gamete containing structure, the male gametophyte or pollen grain. How the dual role of plant miRNAs, regulating both genes and TEs, is integrated during pollen development and which mRNAs are regulated by miRNAs in this cell type at a genome-wide scale are unknown. Here, we provide a detailed analysis of miRNA dynamics and activity during pollen development in Arabidopsis thaliana using small RNA and degradome parallel analysis of RNA end high-throughput sequencing. Furthermore, we uncover miRNAs loaded into the two main active Argonaute (AGO) proteins in the uninuclear and mature pollen grain, AGO1 and AGO5. Our results indicate that the developmental progression from microspore to mature pollen grain is characterized by a transition from miRNAs targeting developmental genes to miRNAs regulating TE activity.sRNA, PARE, and AGO-IP sequencing uncovered the role of miRNAs during pollen development, showing that miRNAs transition from regulating genes involved in development to transposable elements.

Authors/Creators:Oliver Velasco, Cecilia and Annacondia, María Luz and Wang, Zhenxing and Jullien, Pauline E. and Slotkin, R. Keith and Köhler, Claudia and Martinez Arias, German
Title:The miRNome function transitions from regulating developmental genes to transposable elements during pollen maturation
Series Name/Journal:Plant Cell
Year of publishing :2022
Volume:34
Number:2
Page range:784-801
Number of Pages:18
Publisher:OXFORD UNIV PRESS INC
ISSN:1040-4651
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Plant Biotechnology
URN:NBN:urn:nbn:se:slu:epsilon-p-114581
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-114581
Additional ID:
Type of IDID
DOI10.1093/plcell/koab280
Web of Science (WoS)000761460300011
ID Code:27552
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231)
Deposited By: SLUpub Connector
Deposited On:14 Apr 2022 10:25
Metadata Last Modified:14 Apr 2022 10:31

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