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Composition and short-term stability of gut microbiota in lean and spontaneously overweight healthy Labrador retriever dogs

Söder, Josefin and Wernersson, Sara and Höglund, Katja and Hagman, Ragnvi and Truelsen Lindåse, Sanna and Dicksved, Johan (2022). Composition and short-term stability of gut microbiota in lean and spontaneously overweight healthy Labrador retriever dogs. Acta Veterinaria Scandinavica. 64 :1 , 8
[Research article]

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Abstract

Background The gut microbiota and its metabolic end-products act in close collaboration with the nutrient metabolism of the animal. A relationship between excess adiposity and alterations in gut microbiota composition has been identified in humans and rodents, but data are scarce for overweight dogs. This study compared composition and temporal variations of gut microbiota in healthy lean and spontaneously overweight dogs. The analysis was based on three individual fresh faeces samples from each dog during a 10-day period. Twenty-seven healthy and intact male Labrador retriever dogs were included, 12 of which were classified as lean (body condition score (BCS) 4-5 on a 9-point scale) and 15 as overweight (BCS 6-8). Gut microbiota was analysed by Illumina sequencing of the V3-V4 region of the 16S rRNA gene. Results Lean and overweight groups of dogs were not separated by principal coordinate analysis (PCoA), analysis of similarity (one-way ANOSIM, P = 0.99) or species indicator analysis (IndVal) using operational taxonomic units (OTU) data. Gut microbial taxa at phylum, family or genus level did not differ between lean and overweight dogs in mixed-model repeated measures analyses. Short-term stability, evaluated by similarity index, did not differ between lean and overweight dogs over the 10-day period. Pooled Firmicutes/Bacteroidetes (F/B) ratio was 3.1 +/- 3.7 in overweight dogs and 2.1 +/- 1.2 in lean dogs (P = 0.83). Individual dogs, irrespective of body condition (lean or overweight), displayed variation in mean alpha diversity (Chao-1 index range 122-245, Shannon index range 2.6-3.6) and mean similarity index (range 44-85%). Conclusions Healthy lean and spontaneously overweight Labrador retriever dogs had comparable gut microbiota composition and short-term stability over a 10-day sampling period. There were no alterations in microbial diversity or in relative abundance of specific taxa at phylum, family or genus level in overweight compared to lean dogs. Our findings suggest that there are few detectable differences in gut microbiota composition between healthy spontaneously overweight and lean dogs by the current method. Future application of metagenomic or metabolomic techniques could be used to investigate microbial genes or microbial end-products that may differ even when microbiota compositional analyses fail to detect a significant difference between lean and overweight dogs.

Authors/Creators:Söder, Josefin and Wernersson, Sara and Höglund, Katja and Hagman, Ragnvi and Truelsen Lindåse, Sanna and Dicksved, Johan
Title:Composition and short-term stability of gut microbiota in lean and spontaneously overweight healthy Labrador retriever dogs
Series Name/Journal:Acta Veterinaria Scandinavica
Year of publishing :2022
Volume:64
Number:1
Article number:8
Number of Pages:9
Publisher:BMC
ISSN:0044-605X
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
Keywords:16S rRNA sequencing, Canine, Faeces sampling, Obesity, Temporal variations
URN:NBN:urn:nbn:se:slu:epsilon-p-116743
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-116743
Additional ID:
Type of IDID
DOI10.1186/s13028-022-00628-z
Web of Science (WoS)000773942900001
ID Code:27598
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
(VH) > Dept. of Anatomy, Physiology and Biochemistry
(VH) > Dept. of Animal Nutrition and Management
Deposited By: SLUpub Connector
Deposited On:22 Apr 2022 15:25
Metadata Last Modified:22 Apr 2022 15:31

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