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Detection and isolation of airborne SARS-CoV-2 in a hospital setting

de Sousa, Nuno Rufino and Steponaviciute, Laura and Margerie, Lucille and Nissen, Karolina and Kjellin, Midori and Reinius, Bjorn and Salaneck, Erik and Udekwu, Klas and Rothfuchs, Antonio Gigliotti (2022). Detection and isolation of airborne SARS-CoV-2 in a hospital setting. Indoor Air. 32 :3 , e13023
[Research article]

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Abstract

Transmission mechanisms for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are incompletely understood. In particular, aerosol transmission remains unclear, with viral detection in air and demonstration of its infection potential being actively investigated. To this end, we employed a novel electrostatic collector to sample air from rooms occupied by COVID-19 patients in a major Swedish hospital. Electrostatic air sampling in conjunction with extraction-free, reverse-transcriptase polymerase chain reaction (hid-RT-PCR) enabled detection of SARS-CoV-2 in air from patient rooms (9/22; 41%) and adjoining anterooms (10/22; 45%). Detection with hid-RT-PCR was concomitant with viral RNA presence on the surface of exhaust ventilation channels in patients and anterooms more than 2 m from the COVID-19 patient. Importantly, it was possible to detect active SARS-CoV-2 particles from room air, with a total of 496 plaque-forming units (PFUs) being isolated, establishing the presence of infectious, airborne SARS-CoV-2 in rooms occupied by COVID-19 patients. Our results support circulation of SARS-CoV-2 via aerosols and urge the revision of existing infection control frameworks to include airborne transmission.

Authors/Creators:de Sousa, Nuno Rufino and Steponaviciute, Laura and Margerie, Lucille and Nissen, Karolina and Kjellin, Midori and Reinius, Bjorn and Salaneck, Erik and Udekwu, Klas and Rothfuchs, Antonio Gigliotti
Title:Detection and isolation of airborne SARS-CoV-2 in a hospital setting
Series Name/Journal:Indoor Air
Year of publishing :2022
Volume:32
Number:3
Article number:e13023
Number of Pages:8
Publisher:WILEY
ISSN:0905-6947
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 3 Medical and Health Sciences > 303 Health Sciences > Environmental Health and Occupational Health
Keywords:environmental sampling, health care, infectious aerosols, pathogen detection, SARS-CoV-2, transmission
URN:NBN:urn:nbn:se:slu:epsilon-p-116747
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-116747
Additional ID:
Type of IDID
DOI10.1111/ina.13023
Web of Science (WoS)000773439100001
ID Code:27606
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Aquatic Sciences and Assessment
Deposited By: SLUpub Connector
Deposited On:25 Apr 2022 08:25
Metadata Last Modified:25 Apr 2022 08:31

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