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Release of moth pheromone compounds from Nicotiana benthamiana upon transient expression of heterologous biosynthetic genes

Xia, Yi-Han and Ding, Bao-Jian and Dong, Shuang-Lin and Wang, Hong-Lei and Hofvander, Per and Lofstedt, Christer (2022). Release of moth pheromone compounds from Nicotiana benthamiana upon transient expression of heterologous biosynthetic genes. BMC Biology. 20 :1 , 80
[Research article]

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Abstract

Background Using genetically modified plants as natural dispensers of insect pheromones may eventually become part of a novel strategy for integrated pest management. Results In the present study, we first characterized essential functional genes for sex pheromone biosynthesis in the rice stem borer Chilo suppressalis (Walker) by heterologous expression in Saccharomyces cerevisiae and Nicotiana benthamiana, including two desaturase genes CsupYPAQ and CsupKPSE and a reductase gene CsupFAR2. Subsequently, we co-expressed CsupYPAQ and CsupFAR2 together with the previously characterized moth desaturase Atr increment 11 in N. benthamiana. This resulted in the production of (Z)-11-hexadecenol together with (Z)-11-hexadecenal, the major pheromone component of C. suppressalis. Both compounds were collected from the transformed N. benthamiana headspace volatiles using solid-phase microextraction. We finally added the expression of a yeast acetyltransferase gene ATF1 and could then confirm also (Z)-11-hexadecenyl acetate release from the plant. Conclusions Our results pave the way for stable transformation of plants to be used as biological pheromone sources in different pest control strategies.

Authors/Creators:Xia, Yi-Han and Ding, Bao-Jian and Dong, Shuang-Lin and Wang, Hong-Lei and Hofvander, Per and Lofstedt, Christer
Title:Release of moth pheromone compounds from Nicotiana benthamiana upon transient expression of heterologous biosynthetic genes
Series Name/Journal:BMC Biology
Year of publishing :2022
Volume:20
Number:1
Article number:80
Number of Pages:18
Publisher:BMC
ISSN:1741-7007
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Plant Biotechnology
Keywords:Functional characterization, Fatty acyl desaturases, Fatty acyl reductase, Alcohol oxidation, Acetyltransferase, Heterologous expression systems, Pheromone-releasing plants
URN:NBN:urn:nbn:se:slu:epsilon-p-116776
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-116776
Additional ID:
Type of IDID
DOI10.1186/s12915-022-01281-8
Web of Science (WoS)000776736100002
ID Code:27635
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Breeding (from 130101)
Deposited By: SLUpub Connector
Deposited On:26 Apr 2022 13:25
Metadata Last Modified:26 Apr 2022 13:31

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